Evidence map›Paper›PMID 21673099›Full record

ArticleEndocrinology2011

1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells.

Leah A Garcia, Keisha K King, Monica G Ferrini, Keith C Norris, Jorge N Artaza

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
125citing papers in PubMed, 5 pooled it
10.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

125 citing papers in PubMed, 5 syntheses or guidelines pooled it, 255 citations in OpenAlex.

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65 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Leah A GarciaDepartment of Internal Medicine, Charles R. Drew University of Medicine and Science, 1731 East 120th Street, Los Angeles, California 90059, USA.
Keisha K King
Monica G Ferrini
Keith C Norris
Jorge N Artaza
Charles R. Drew University of Medicine and Science · USUniversity of California, Los Angeles · US

Funding

Sustaining Faculty Development & Community EngagementS21MD000103 · NIMHD · CHARLES R. DREW UNIVERSITY OF MED &SCI · PI CARLISLE, DAVID M · 2001 to 2015
$73.9M
THE NUTRIGENOMICS OF VITAMIN D METABOLISM IN AFRICAN-AMERICANSU54RR022762 · NCRR · CHARLES R. DREW UNIVERSITY OF MED & SCI · PI NORRIS, KEITH C · 2007 to 2011
$19.6M
TRAINING COREP20MD000182 · NIMHD · CHARLES R. DREW UNIVERSITY OF MED &SCI · PI CUNNINGHAM, WILLIAM EMERY, SHAPIRO, MARTIN F. · 2002 to 2016
$16.5M
VITAMIN D STIMULATES CARDIAC DIFFERENTIATIONU54RR026138 · NCRR · CHARLES R. DREW UNIVERSITY OF MED & SCI · PI NORRIS, KEITH C · 2009 to 2011
$14.0M
Nitric Oxide/cGMP Modulation of Corporal Fibrosis Caused by NeuropraxiaSC1NS064611 · NINDS · CHARLES R. DREW UNIVERSITY OF MED & SCI · PI FERRINI, MONICA GABRIELA · 2008 to 2012
$1.4M
NCRR NIH HHS RR022762NCRR NIH HHS RR026138NCRR NIH HHS U54 RR022762NCRR NIH HHS U54 RR026138NIMHD NIH HHS MD000103NIMHD NIH HHS MD000182NIMHD NIH HHS P20 MD000182NIMHD NIH HHS S21 MD000103NINDS NIH HHS SC1 NS064611NINDS NIH HHS SC1NS064611
6 · The paper itself

Abstract

Skeletal muscle wasting is an important public health problem associated with aging, chronic disease, cancer, kidney dialysis, and HIV/AIDS. 1,25-Dihydroxyvitamin D (1,25-D3), the active form of vitamin D, is widely recognized for its regulation of calcium and phosphate homeostasis in relation to bone development and maintenance and for its calcemic effects on target organs, such as intestine, kidney, and parathyroid glands. Emerging evidence has shown that vitamin D administration improves muscle performance and reduces falls in vitamin D-deficient older adults. However, little is known of the underlying mechanism or the role 1,25-D3 plays in promoting myogenic differentiation at the cellular and/or molecular level. In this study, we examined the effect of 1,25-D3 on myoblast cell proliferation, progression, and differentiation into myotubes. C(2)C(12) myoblasts were treated with 1,25-D3 or placebo for 1, 3, 4, 7, and 10 d. Vitamin D receptor expression was analyzed by quantitative RT-PCR, Western blottings and immunofluorescence. Expression of muscle lineage, pro- and antimyogenic, and proliferation markers was assessed by immunocytochemistry, PCR arrays, quantitative RT-PCR, and Western blottings. Addition of 1,25-D3 to C(2)C(12) myoblasts 1) increased expression and nuclear translocation of the vitamin D receptor, 2) decreased cell proliferation, 3) decreased IGF-I expression, and 4) promoted myogenic differentiation by increasing IGF-II and follistatin expression and decreasing the expression of myostatin, the only known negative regulator of muscle mass, without changing growth differentiation factor 11 expression. This study identifies key vitamin D-related molecular pathways for muscle regulation and supports the rationale for vitamin D intervention studies in select muscle disorder conditions.

Indexed as

Active Transport, Cell NucleusAnimalsBone Morphogenetic ProteinsCalcitriolCell ProliferationCells, CulturedGene Expression RegulationGrowth Differentiation FactorsInsulin-Like Growth Factor IIMiceMuscle DevelopmentMuscle, SkeletalMyoD ProteinMyostatinReceptors, CalcitriolBone Morphogenetic ProteinsCalcitriolGdf11 protein, mouseGrowth Differentiation FactorsInsulin-Like Growth Factor IIMstn protein, mouseMyoD1 myogenic differentiation proteinMyoD ProteinMyostatinReceptors, Calcitriol

Identifiers

PMID21673099
PMCPMC3138228
OpenAlexW2070216811

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.