ArticleBMC microbiology2011
Prevalence, distribution and evolutionary significance of the IS629 insertion element in the stepwise emergence of Escherichia coli O157:H7.
Article in BMC microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Comparative Genomics ofMicroorganisms · 2022Article
- Phylogeny, Prevalence, and Shiga Toxin (Stx) Production of Clinical Escherichia coli O157 Clade 2 Strains Isolated in Shimane Prefecture, Japan.Current microbiology · 2021Article
- Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation.BMC genomics · 2018Article
- Virulence Gene Profiles and Clonal Relationships of Escherichia coli O26:H11 Isolates from Feedlot Cattle as Determined by Whole-Genome Sequencing.Applied and environmental microbiology · 2016Article
- Simultaneous Presence of Insertion Sequence Excision Enhancer and Insertion Sequence IS629 Correlates with Increased Diversity and Virulence in Shiga Toxin-Producing Escherichia coli.Journal of clinical microbiology · 2015Article
- Distribution of IS629 and stx genotypes among enterohemorrhagic Escherichia coli O157 isolates in Yamaguchi Prefecture, Japan, 2004-2013.The Journal of veterinary medical science · 2015Article
- Comparative genomics of enterohemorrhagic Escherichia coli O145:H28 demonstrates a common evolutionary lineage with Escherichia coli O157:H7.BMC genomics · 2014Article
- Complete DNA sequence analysis of enterohemorrhagic Escherichia coli plasmid pO157_2 in β-glucuronidase-positive E. coli O157:H7 reveals a novel evolutionary path.Journal of bacteriology · 2012Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInsertion elements (IS) are known to play an important role in the evolution and genomic diversification of Escherichia coli O157:H7 lineages. In particular, IS629 has been found in multiple copies in the E. coli O157:H7 genome and is one of the most prevalent IS in this serotype. It was recently shown that the lack of O157 antigen expression in two O rough E. coli O157:H7 strains was due to IS629 insertions at 2 different locations in the gne gene that is essential for the O antigen biosynthesis.
resultsThe comparison of 4 E. coli O157:H7 genome and plasmid sequences showed numerous IS629 insertion sites, although not uniformly distributed among strains. Comparison of IS629s found in O157:H7 and O55:H7 showed the presence of at least three different IS629 sub-types. O157:H7 strains carry IS629 elements sub-type I and III whereby the ancestral O55:H7 carries sub-type II. Analysis of strains selected from various clonal groups defined on the E. coli O157:H7 stepwise evolution model showed that IS629 was not observed in sorbitol fermenting O157 (SFO157) clones that are on a divergent pathway in the emergence of O157:H7. This suggests that the absence of IS629 in SFO157 strains probably occurred during the divergence of this lineage, albeit it remains uncertain if it contributed, in part, to their divergence from other closely related strains.
conclusionsThe highly variable genomic locations of IS629 in O157:H7 strains of the A6 clonal complex indicates that this insertion element probably played an important role in genome plasticity and in the divergence of O157:H7 lineages.
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