ArticleJournal of the American Chemical Society2011
Enhancing protease activity assay in droplet-based microfluidics using a biomolecule concentrator.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Evaluation of single-cell heterogeneity and invasive potential in cancer cells via secreted protease activity assay.Analytica chimica acta · 2025Article
- Simulation and Experimental Study of Ion Concentration Polarization Induced Electroconvective Vortex and Particle Movement.Micromachines · 2021Article
- Rapid Detection of β-Lactamase-Producing Bacteria Using the Integrated Comprehensive Droplet Digital Detection (IC 3D) System.Sensors (Basel, Switzerland) · 2020Article
- Direct Visualization of Perm-Selective Ion Transportation.Scientific reports · 2020Article
- Giant magnetoresistive biosensors for real-time quantitative detection of protease activity.Scientific reports · 2020Article
- Nanoelectrokinetic bufferchannel-less radial preconcentrator and online extractor by tunable ion depletion layer.Biomicrofluidics · 2019Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The study of atmospheric ice-nucleating particles via microfluidically generated droplets.Microfluidics and nanofluidics · 2018Article
- Multivesicular droplets: a cell model system to study compartmentalised biochemical reactions.Lab on a chip · 2017Article
- Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone.Journal of visualized experiments : JoVE · 2017Article
- Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis.Molecules (Basel, Switzerland) · 2016Review
- High-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidics.Scientific reports · 2016Article
- Capillarity ion concentration polarization for spontaneous biomolecular preconcentration mechanism.Biomicrofluidics · 2016Article
- Floating Droplet Array: An Ultrahigh-Throughput Device for Droplet Trapping, Real-time Analysis and Recovery.Micromachines · 2015Article
- Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions.RNA (New York, N.Y.) · 2015Article
- Real-time measurement of thrombin generation using continuous droplet microfluidics.Biomicrofluidics · 2014Article
- Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis.Journal of the American Chemical Society · 2013Article
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.