ArticleProceedings of the National Academy of Sciences of the United States of America2011
Metabolomic analysis of Arabidopsis reveals hemiterpenoid glycosides as products of a nitrate ion-regulated, carbon flux overflow.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Isopentenyl diphosphate isomerase exerts limited control over terpenoid biosynthesis in two woody plant species.Plant physiology · 2026Article
- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- The methylerythritol phosphate pathway as an oxidative stress sense and response system.Nature communications · 2024Review
- Deoxyxylulose 5-Phosphate Synthase Does Not Play a Major Role in Regulating the Methylerythritol 4-Phosphate Pathway in Poplar.International journal of molecular sciences · 2024Article
- Article
- Comparative transcriptome analysis provides insights into steviol glycoside synthesis in stevia (Stevia rebaudiana Bertoni) leaves under nitrogen deficiency.Plant cell reports · 2021Article
- Physiological and Biochemical Traits of Two MajorFrontiers in plant science · 2021Article
- Plant Volatile Organic Compounds Evolution: Transcriptional Regulation, Epigenetics and Polyploidy.International journal of molecular sciences · 2020Review
- Lethal heat stress-dependent volatile emissions from tobacco leaves: what happens beyond the thermal edge?Journal of experimental botany · 2019Article
- Alternative Carbon Sources for Isoprene Emission.Trends in plant science · 2018Review
- Accumulation of Carotenoids and Metabolic Profiling in Different Cultivars of Tagetes Flowers.Molecules (Basel, Switzerland) · 2017Article
- Induction of Terpene Biosynthesis in Berries of Microvine Transformed withFrontiers in plant science · 2017Article
- Artificially decreased vapour pressure deficit in field conditions modifies foliar metabolite profiles in birch and aspen.Journal of experimental botany · 2016Article
- Glycosylation Is a Major Regulator of Phenylpropanoid Availability and Biological Activity in Plants.Frontiers in plant science · 2016Review
- Relative mass defect filtering of mass spectra: a path to discovery of plant specialized metabolites.Plant physiology · 2015Article
- Nitrogen Limitation Alters Biomass Production but Enhances Steviol Glycoside Concentration in Stevia rebaudiana Bertoni.PloS one · 2015Article
- An efficient high throughput metabotyping platform for screening of biomass willows.Metabolites · 2014Article
- Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis.Plant physiology · 2014Article
- A genome-wide metabolomic resource for tomato fruit from Solanum pennellii.Scientific reports · 2014Article
- Cell metabolomics.Omics : a journal of integrative biology · 2013Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
An understanding of the balance between carbon and nitrogen assimilation in plants is key to future bioengineering for a range of applications. Metabolomic analysis of the model plant, Arabidopsis thaliana, using combined NMR-MS revealed the presence of two hemiterpenoid glycosides that accumulated in leaf tissue, to ~1% dry weight under repeated nitrate-deficient conditions. The formation of these isoprenoids was correlated with leaf nitrate concentrations that could also be assayed in the metabolomic data using a unique flavonoid-nitrate mass spectral adduct. Analysis of leaf and root tissue from plants grown in hydroponics with a variety of root stressors identified the conditions under which the isoprenoid pathway in leaves was diverted to the hemiterpenoids. These compounds were strongly induced by root wounding or oxidative stress and weakly induced by potassium deficiency. Other stresses such as cold, saline, and osmotic stress did not induce the compounds. Replacement of nitrate with ammonia failed to suppress the formation of the hemiterpenoids, indicating that nitrate sensing was a key factor. Feeding of intermediates was used to study aspects of 2-C-methyl-d-erythritol-4-phosphate pathway regulation leading to hemiterpenoid formation. The formation of the hemiterpenoids in leaves was strongly correlated with the induction of the phenylpropanoids scopolin and coniferin in roots of the same plants. These shunts of photosynthetic carbon flow are discussed in terms of overflow mechanisms that have some parallels with isoprene production in tree species.
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