ArticleProceedings of the National Academy of Sciences of the United States of America2011
In vitro assembly of physiological cohesin/DNA complexes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Identification and characterization of a human MORC2 DNA binding region that is required for gene silencing.Nucleic acids research · 2025Article
- Identification and characterization of a human MORC2 DNA binding region that is required for gene silencing.bioRxiv : the preprint server for biology · 2024Article
- Article
- It's all in the numbers: Cohesin stoichiometry.Frontiers in molecular biosciences · 2022Review
- Article
- Intermediate step of cohesin's ATPase cycle allows cohesin to entrap DNA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Of Rings and Rods: Regulating Cohesin Entrapment of DNA to Generate Intra- and Intermolecular Tethers.PLoS genetics · 2016Review
- Single-Molecule Imaging Reveals a Collapsed Conformational State for DNA-Bound Cohesin.Cell reports · 2016Article
- The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.eLife · 2015Article
- Article
- Cell cycle-specific cleavage of Scc2 regulates its cohesin deposition activity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mechanisms of cohesin-mediated gene regulation and lessons learned from cohesinopathies.Biochimica et biophysica acta · 2014Review
- Article
- Cohesin-based chromatin interactions enable regulated gene expression within preexisting architectural compartments.Genome research · 2013Article
- The SMC1-SMC3 cohesin heterodimer structures DNA through supercoiling-dependent loop formation.Nucleic acids research · 2013Article
- Cohesin codes - interpreting chromatin architecture and the many facets of cohesin function.Journal of cell science · 2013Review
- Chl1 DNA helicase regulates Scc2 deposition specifically during DNA-replication in Saccharomyces cerevisiae.PloS one · 2013Article
- In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Cohesin is a member of the Smc family of protein complexes that mediates higher-order chromosome structure by tethering different regions of chromatin. We present a new in vitro system that assembles cohesin-DNA complexes with in vivo properties. The assembly of these physiological salt-resistant complexes requires the cohesin holo-complex, its ability to bind ATP, the cohesin loader Scc2p and a closed DNA topology. Both the number of cohesin molecules bound to the DNA substrate and their distribution on the DNA substrate are limited. Cohesin and Scc2p bind preferentially to cohesin associated regions (CARs), DNA sequences with enriched cohesin binding in vivo. A subsequence of CARC1 promotes cohesin binding to neighboring sequences within CARC1. The enhancer-like function of this sequence is validated by in vivo deletion analysis. By demonstrating the physiological relevance of these in vitro assembled cohesin-DNA complexes, we establish our in vitro system as a powerful tool to elucidate the mechanism of cohesin and other Smc complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.