ArticlePlant physiology2011
Proline accumulation is inhibitory to Arabidopsis seedlings during heat stress.
Article in Plant physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 232 citations in OpenAlex.
- Acidic and Alkaline pH Stresses Impair Tomato Seed Germination and Seedling Growth via Disruption of Reactive Oxygen Species and Auxin Homeostasis.Plants (Basel, Switzerland) · 2026Article
- Beyond osmoprotection: the expanding roles of proline in plant signalling and development.Biologia futura · 2026Review
- Decreases in polyunsaturated fatty acid content improve heat stress tolerance during flowering and silicle development in pennycress (Frontiers in plant science · 2026Article
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- Differential biochemical responses of seven Indian wheat genotypes to temperature stress.BMC plant biology · 2025Article
- Transcriptomics and metabolomics analyses of Rosa hybrida to identify heat stress response genes and metabolite pathways.BMC plant biology · 2024Article
- Analysis of the Mechanism of Wood Vinegar and Butyrolactone Promoting Rapeseed Growth and Improving Low-Temperature Stress Resistance Based on Transcriptome and Metabolomics.International journal of molecular sciences · 2024Article
- The Small Auxin-Up RNA 50 (SAUR50) Gene fromPlants (Basel, Switzerland) · 2024Article
- An ecotype-specific effect of osmopriming and melatonin during salt stress in Arabidopsis thaliana.BMC plant biology · 2024Article
- Introgression of ΔTransgenic research · 2024Article
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- Appraisal of the Role of Gaseous Signaling Molecules in Thermo-Tolerance Mechanisms in Plants.Plants (Basel, Switzerland) · 2024Review
- Physiological and biochemical study of the drought tolerance of 14 main olive cultivars in the Mediterranean basin.Photosynthesis research · 2024Article
- Impact of exogenous rhamnolipids on plant photosynthesis and biochemical parameters under prolonged heat stress.Photosynthetica · 2024Article
- Overexpression of the potatoFrontiers in plant science · 2024Article
- Jasmonic acid is required for tomato acclimation to multifactorial stress combination.Environmental and experimental botany · 2023Article
- Plant Metabolomics: An Overview of the Role of Primary and Secondary Metabolites against Different Environmental Stress Factors.Life (Basel, Switzerland) · 2023Review
- Growth and Molecular Responses of Tomato to Prolonged and Short-Term Heat Exposure.International journal of molecular sciences · 2023Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of proline (Pro) accumulation on heat sensitivity was investigated using transgenic Arabidopsis (Arabidopsis thaliana) plants ectopically expressing the Δ(1)-pyrroline-5-carboxylate synthetase 1 gene (AtP5CS1) under the control of a heat shock protein 17.6II gene promoter. During heat stress, the heat-inducible expression of the AtP5CS1 transgene was capable of enhancing Pro biosynthesis. Twelve-day-old seedlings were first treated with heat at 37 °C for 24 h to induce Pro and then were stressed at 50 °C for 4 h. After recovery at 22 °C for 96 h, the growth of Pro-overproducing plants was significantly more inhibited than that of control plants that do not accumulate Pro, manifested by lower survival rate, higher ion leakage, higher reactive oxygen species (ROS) and malondialdehyde levels, and increased activity of the Pro/P5C cycle. The activities of antioxidant enzymes superoxide dismutase, guaiacol peroxidase, and catalase, but not those of glutathione reductase and ascorbate peroxidase, increased in all lines after heat treatment, but the increase was more significant in Pro-overproducing seedlings. Staining with MitoSox-Red, reported for being able to specifically detect superoxide formed in mitochondria, showed that Pro accumulation during heat stress resulted in elevated levels of ROS in mitochondria. Interestingly, exogenous abscisic acid (ABA) and ethylene were found to partially rescue the heat-sensitive phenotype of Pro-overproducing seedlings. Measurement of ethylene and ABA levels further confirmed that these two hormones are negatively affected in Pro-overproducing seedlings during heat stress. Our results indicated that Pro accumulation under heat stress decreases the thermotolerance, probably by increased ROS production via the Pro/P5C cycle and inhibition of ABA and ethylene biosynthesis.
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