ArticleJournal of the American Chemical Society2011
Hydrogen-bonding capability of a templating difluorotoluene nucleotide residue in an RB69 DNA polymerase ternary complex.
Article in Journal of the American Chemical Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Pol γ possesses separate metal binding sites for polymerase and strand displacement functions.Nucleic acids research · 2026Article
- RB69 DNA polymerase structure, kinetics, and fidelity.Biochemistry · 2014Review
- Alteration in the cavity size adjacent to the active site of RB69 DNA polymerase changes its conformational dynamics.Nucleic acids research · 2013Article
- DNA mismatch synthesis complexes provide insights into base selectivity of a B family DNA polymerase.Journal of the American Chemical Society · 2013Article
- Contribution of partial charge interactions and base stacking to the efficiency of primer extension at and beyond abasic sites in DNA.Biochemistry · 2012Article
- Expanding the horizon of the thymine isostere biochemistry: unique cyclobutane dimers formed by photoreaction between a thymine and a toluene residue in the dinucleotide framework.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Article
- Using a fluorescent cytosine analogue tC(o) to probe the effect of the Y567 to Ala substitution on the preinsertion steps of dNMP incorporation by RB69 DNA polymerase.Biochemistry · 2012Article
- Probing minor groove hydrogen bonding interactions between RB69 DNA polymerase and DNA.Biochemistry · 2012Article
- Structural basis for differential insertion kinetics of dNMPs opposite a difluorotoluene nucleotide residue.Biochemistry · 2012Article
- Measurement and theory of hydrogen bonding contribution to isosteric DNA base pairs.Journal of the American Chemical Society · 2012Article
- Synthetic nucleotides as probes of DNA polymerase specificity.Journal of nucleic acids · 2012Article
- Structural insights into complete metal ion coordination from ternary complexes of B family RB69 DNA polymerase.Biochemistry · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Results obtained using 2,4-difluorotoluene nucleobase (dF) as a nonpolar thymine isostere by Kool and colleagues challenged the Watson-Crick dogma that hydrogen bonds between complementary bases are an absolute requirement for accurate DNA replication. Here, we report crystal structure of an RB69 DNA polymerase L561A/S565G/Y567A triple mutant ternary complex with a templating dF opposite dTTP at 1.8 Å-resolution. In this structure, direct hydrogen bonds were observed between: (i) dF and the incoming dTTP, (ii) dF and residue G568 of the polymerase, and (iii) dF and ordered water molecules surrounding the nascent base pair. Therefore, this structure provides evidence that a templating dF can form novel hydrogen bonds with the incoming dTTP and with the enzyme that differ from those formed with a templating dT.
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Registered trials
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