ArticleAnalytical chemistry2011
Ion mobility separation of isomeric phosphopeptides from a protein with variant modification of adjacent residues.
Article in Analytical chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Considerations for defining +80 Da mass shifts in mass spectrometry-based proteomics: phosphorylation and beyond.Chemical communications (Cambridge, England) · 2023Review
- Separation of Isomeric Tau Phosphopeptides from Alzheimer's Disease Brain by Cyclic Ion Mobility Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Article
- Global Phosphoproteome Analysis Using High-Field Asymmetric Waveform Ion Mobility Spectrometry on a Hybrid Orbitrap Mass Spectrometer.Analytical chemistry · 2020Article
- A new azobenzene-based design strategy for detergents in membrane protein research.Chemical science · 2020Article
- Phosphopeptide Fragmentation and Site Localization by Mass Spectrometry: An Update.Analytical chemistry · 2019Review
- A Novel Differential Ion Mobility Device Expands the Depth of Proteome Coverage and the Sensitivity of Multiplex Proteomic Measurements.Molecular & cellular proteomics : MCP · 2018Article
- Linear and Differential Ion Mobility Separations of Middle-Down Proteoforms.Analytical chemistry · 2018Article
- Characterization of Complete Histone Tail Proteoforms Using Differential Ion Mobility Spectrometry.Analytical chemistry · 2017Article
- A Priori Intrinsic PTM Size Parameters for Predicting the Ion Mobilities of Modified Peptides.Journal of the American Society for Mass Spectrometry · 2017Article
- Separation of cis and trans Isomers of Polyproline by FAIMS Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Localization of Post-Translational Modifications in Peptide Mixtures via High-Resolution Differential Ion Mobility Separations Followed by Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2016Article
- To What Extent is FAIMS Beneficial in the Analysis of Proteins?Journal of the American Society for Mass Spectrometry · 2016Article
- Automated deconvolution of overlapped ion mobility profiles.Journal of the American Society for Mass Spectrometry · 2014Article
- Description of gas-phase ion/neutral interactions in differential ion mobility spectrometry: CV prediction using calibration runs.Journal of the American Society for Mass Spectrometry · 2014Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- High-resolution differential ion mobility spectrometry of a protein.Analytical chemistry · 2013Article
- Pushing the Frontier of High-Definition Ion Mobility Spectrometry Using FAIMS.Mass spectrometry (Tokyo, Japan) · 2013Article
- High-field asymmetric waveform ion mobility spectrometry for mass spectrometry-based proteomics.Expert review of proteomics · 2012Review
- Separation of variant methylated histone tails by differential ion mobility.Analytical chemistry · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Ion mobility spectrometry (IMS), and particularly differential or field asymmetric waveform IMS (FAIMS), was recently shown capable of separating peptides with variant localization of post-translational modifications. However, that work was limited to a model peptide with Ser phosphorylation on fairly distant alternative sites. Here, we demonstrate that FAIMS (coupled to electrospray/mass spectrometry (ESI/MS)) can broadly baseline-resolve variant phosphopeptides from a biologically modified human protein, including those involving phosphorylation of different residues and adjacent sites that challenge existing tandem mass spectrometry (MS/MS) methods most. Singly and doubly phosphorylated variants can be resolved equally well and identified without dissociation, based on accurate separation properties. The spectra change little over a range of infusion solvent pH; hence, the present approach should be viable in conjunction with chromatographic separations using mobile phase gradients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.