ArticleMolecular biology reports2012
Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- RNAi-Mediated Resistance Against Viruses in Perennial Fruit Plants.Plants (Basel, Switzerland) · 2019Review
- Genome-Wide Profiling of Small RNAs and Degradome Revealed Conserved Regulations of miRNAs on Auxin-Responsive Genes during Fruit Enlargement in Peaches.International journal of molecular sciences · 2017Article
- Computational prediction of candidate miRNAs and their potential functions in biomineralization in pearl oyster Pinctada martensii.Saudi journal of biological sciences · 2016Article
- miR-29a Participated in Nacre Formation and Immune Response by Targeting Y2R in Pinctada martensii.International journal of molecular sciences · 2015Article
- Role of bioinformatics in establishing microRNAs as modulators of abiotic stress responses: the new revolution.Frontiers in physiology · 2015Review
- Identification of chilling stress-responsive tomato microRNAs and their target genes by high-throughput sequencing and degradome analysis.BMC genomics · 2014Article
- Identification and characterization of microRNAs in pearl oyster Pinctada martensii by Solexa deep sequencing.Marine biotechnology (New York, N.Y.) · 2014Article
- Computational identification of conserved microRNAs and their targets from expression sequence tags of blueberry (Vaccinium corybosum).Plant signaling & behavior · 2014Article
- Characterization and expression profiling of selected microRNAs in tomato (Solanum lycopersicon) 'Jiangshu14'.Molecular biology reports · 2013Article
- Computational Identification of MicroRNAs from the Expressed Sequence Tags of Toxic Dinoflagellate Alexandrium Tamarense.Evolutionary bioinformatics online · 2013Article
- Integrative analysis of miRNA and mRNA profiles in response to ethylene in rose petals during flower opening.PloS one · 2013Article
- Identification of miRNAs and their target genes in peach (Prunus persica L.) using high-throughput sequencing and degradome analysis.PloS one · 2013Article
- The prediction of the porcine pre-microRNAs in genome-wide based on support vector machine (SVM) and homology searching.BMC genomics · 2012Article
- MicroRNAs in trees.Plant molecular biology · 2012Review
- Identification and validation of potential conserved microRNAs and their targets in peach (Prunus persica).Molecules and cells · 2012Article
- Unique expression, processing regulation, and regulatory network of peach (Prunus persica) miRNAs.BMC plant biology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Twenty-two potential miRNAs from seven miRNA families were first predicted from more than 80,857 EST sequences of peach (Prunus persica). Using two specific 5' and 3' miRNA RACE (miR-RACE) PCR reactions and sequence-directed cloning, we accurately determined the precise sequences, especially both ends, of eight candidate miRNAs. The sequencing results demonstrated that the ppe-miRNAs were conserved to those that were predicted computationally except ppe-miR171b. We validated the existence of two members (ppe-miR171a and miR171b) of the miR171 family in peach that belonged to different precursors. qRT-PCR was further employed in analyzing expression of the eight miRNAs in peach leaves, flowers, and fruits at different developing stages, where some of the miRNAs showed tissue-specific expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.