ArticleJournal of assisted reproduction and genetics2011
Digital holographic microscopy in human sperm imaging.
Article in Journal of assisted reproduction and genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos†.Biology of reproduction · 2024Review
- Human live spermatozoa morphology assessment using digital holographic microscopy.Scientific reports · 2022Article
- Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.Scientific reports · 2019Article
- Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress condition.Scientific reports · 2019Article
- Review
- 4D tracking of clinical seminal samples for quantitative characterization of motility parameters.Biomedical optics express · 2014Article
- Article
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Authors and funding
6 authors.
Funding
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Abstract
purposeThe aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT).
methodsDHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested.
resultsIn NZ samples median phase shifts were in the range 2.72-3.21 rad and 2.00-2.15 in OAT samples. Differences among individual samples were statistically significant (p < 0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p < 0.001).
conclusionQuantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility.
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