ArticleAmerican journal of physiology. Heart and circulatory physiology2011
Visualizing calcium responses to acetylcholine convection along endothelium of arteriolar networks in Cx40BAC-GCaMP2 transgenic mice.
Article in American journal of physiology. Heart and circulatory physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 25 citations in OpenAlex.
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- The Emerging Role of Endothelial Ion Channels in the Control of Human Microcirculation.International journal of molecular sciences · 2026Review
- Electrocalcium coupling in brain capillaries: Rapidly traveling electrical signals ignite local calcium signals.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Endothelial calcium dynamics elicited by ATP release from red blood cells.Scientific reports · 2024Article
- The conducted vasomotor response and the principles of electrical communication in resistance arteries.Physiological reviews · 2024Review
- Cracking the Endothelial Calcium (CaInternational journal of molecular sciences · 2023Review
- Computer-assisted image analysis of agonist-mediated microvascular constriction response in mouse cremaster muscle.PloS one · 2022Article
- The Calcium Signaling Mechanisms in Arterial Smooth Muscle and Endothelial Cells.Comprehensive Physiology · 2021Article
- Calcium Signal Profiles in Vascular Endothelium from Cdh5-GCaMP8 and Cx40-GCaMP2 Mice.Journal of vascular research · 2021Article
- VEGF-A inhibits agonist-mediated CaThe Journal of physiology · 2018Article
- Impact of Aging on Calcium Signaling and Membrane Potential in Endothelium of Resistance Arteries: A Role for Mitochondria.The journals of gerontology. Series A, Biological sciences and medical sciences · 2017Article
- All muscarinic acetylcholine receptors (MScientific reports · 2017Article
- Calcium and electrical signaling in arterial endothelial tubes: New insights into cellular physiology and cardiovascular function.Microcirculation (New York, N.Y. : 1994) · 2017Review
- Advanced age decreases local calcium signaling in endothelium of mouse mesenteric arteries in vivo.American journal of physiology. Heart and circulatory physiology · 2016Article
- Decoding dynamic Ca(2+) signaling in the vascular endothelium.Frontiers in physiology · 2014Article
- Vascular tone and Ca(2+) signaling in murine cremaster muscle arterioles in vivo.Microcirculation (New York, N.Y. : 1994) · 2013Article
- Ageing alters perivascular nerve function of mouse mesenteric arteries in vivo.The Journal of physiology · 2013Article
- Coordination of intercellular Ca(2+) signaling in endothelial cell tubes of mouse resistance arteries.Microcirculation (New York, N.Y. : 1994) · 2012Article
- Dynamic Ca(2+) signal modalities in the vascular endothelium.Microcirculation (New York, N.Y. : 1994) · 2012Review
- Characterization of the thoracodorsal artery: morphology and reactivity.Microcirculation (New York, N.Y. : 1994) · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Acetylcholine evokes endothelium-dependent vasodilation subsequent to a rise in intracellular calcium. Despite widespread application in human and animal studies, calcium responses to intravascular ACh have not been visualized in vivo. Microiontophoresis of ACh in tissue adjacent to an arteriole activates abluminal muscarinic receptors on endothelial cells within a "local" region of diffusion, but it is unknown whether ACh released in such fashion gains access to the flow stream resulting in further actions downstream. To test this hypothesis and provide new insight into calcium signaling in vivo, we studied the cremaster muscle microcirculation of anesthetized male Cx40(BAC)-GCaMP2 transgenic mice (n = 22; 5-9 mo; 33 ± 1 g) expressing the fluorescent calcium sensor GCaMP2 selectively in arteriolar endothelial cells. Submaximal ACh stimuli were delivered using microiontophoresis (1-μm pipette tip, 500 nA). With stimulus duration <500 ms or with the micropipette positioned within one vessel diameter (∼30 μm) away from an arteriole, endothelial cell calcium fluorescence was restricted to the region of ACh diffusion (<200 μm). In contrast, with the micropipette tip positioned immediately adjacent to an arteriole or within its lumen, calcium fluorescence encompassed entire networks downstream. The velocity of downstream calcium signaling in response to ACh increased with centerline velocity of fluorescent tracer microbeads (r(2) > 0.99; range: <1 mm/s to >10 mm/s). Diverting arteriolar blood flow into a side branch redirected downstream fluorescence responses to ACh; occluding flow abolished responses. Blocking luminal muscarinic receptors (intravascular glycopyrrolate; 6 μg/kg) inhibited downstream responses reversibly. Through visualizing the actions of a "local" ACh stimulus on endothelial cell calcium fluorescence in vivo, the present findings illustrate that transmural diffusion and convection of an agonist can activate entire networks of arteriolar endothelial cells concomitant with its delivery in the flow stream.
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