ArticleThe Plant cell2011
A guideline to family-wide comparative state-of-the-art quantitative RT-PCR analysis exemplified with a Brassicaceae cross-species seed germination case study.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed.
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- The initiation of embryo growth in imbibed celery mericarps marks a key mechanism by which temperature signals are integrated to regulate germination timing.Journal of experimental botany · 2026Article
- Distinct Molecular Biomechanical Mechanisms Inhibit Endosperm Cell-Wall Weakening and Seed Germination at Cold and Warm Nonoptimal Temperatures.Plant, cell & environment · 2025Article
- The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The dimorphic diaspore model Aethionema arabicum (Brassicaceae): Distinct molecular and morphological control of responses to parental and germination temperatures.The Plant cell · 2024Article
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- Identification of Reference Genes for Precise Expression Analysis during Germination inInternational journal of molecular sciences · 2023Article
- Transcriptome and Physiological Analysis of Rapeseed Tolerance to Post-Flowering Temperature Increase.International journal of molecular sciences · 2023Article
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- Distinct hormonal and morphological control of dormancy and germination inFrontiers in plant science · 2023Article
- Molecular mechanisms of seed dormancy release by gas plasma-activated water technology.Journal of experimental botany · 2022Article
- Gas-Plasma-Activated Water Impact on Photo-Dependent Dormancy Mechanisms inInternational journal of molecular sciences · 2022Article
- The Phytotoxin Myrigalone A Triggers a Phased Detoxification Programme and InhibitsInternational journal of molecular sciences · 2022Article
- Cold-induced secondary dormancy and its regulatory mechanisms in Beta vulgaris.Plant, cell & environment · 2022Article
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- Systematic analysis of differentially expressedPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Transcriptome Analysis of Seed Weight Plasticity inInternational journal of molecular sciences · 2021Article
- Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.The Plant journal : for cell and molecular biology · 2021Article
- The seed water content as a time-independent physiological trait during germination in wild tree species such as Ceiba aesculifolia.Scientific reports · 2020Article
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4 authors.
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Abstract
Comparative biology includes the comparison of transcriptome and quantitative real-time RT-PCR (qRT-PCR) data sets in a range of species to detect evolutionarily conserved and divergent processes. Transcript abundance analysis of target genes by qRT-PCR requires a highly accurate and robust workflow. This includes reference genes with high expression stability (i.e., low intersample transcript abundance variation) for correct target gene normalization. Cross-species qRT-PCR for proper comparative transcript quantification requires reference genes suitable for different species. We addressed this issue using tissue-specific transcriptome data sets of germinating Lepidium sativum seeds to identify new candidate reference genes. We investigated their expression stability in germinating seeds of L. sativum and Arabidopsis thaliana by qRT-PCR, combined with in silico analysis of Arabidopsis and Brassica napus microarray data sets. This revealed that reference gene expression stability is higher for a given developmental process between distinct species than for distinct developmental processes within a given single species. The identified superior cross-species reference genes may be used for family-wide comparative qRT-PCR analysis of Brassicaceae seed germination. Furthermore, using germinating seeds, we exemplify optimization of the qRT-PCR workflow for challenging tissues regarding RNA quality, transcript stability, and tissue abundance. Our work therefore can serve as a guideline for moving beyond Arabidopsis by establishing high-quality cross-species qRT-PCR.
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