ArticleJournal of bacteriology2011
Self-transmissibility of the integrative and conjugative element ICEPm1 between clinical isolates requires a functional integrase, relaxase, and type IV secretion system.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
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- Pangenome Analysis Reveals Novel Contact-Dependent Growth Inhibition System and Phenazine Biosynthesis Operons inMicroorganisms · 2024Article
- Genomic analysis reveals the role of integrative and conjugative elements in plant pathogenic bacteria.Mobile DNA · 2022Article
- Characterization of a Novel Linezolid Resistance GeneMicrobiology spectrum · 2022Article
- Characterization of integrative and conjugative elements carrying antibiotic resistance genes ofFrontiers in microbiology · 2022Article
- Sequence analysis of tyrosine recombinases allows annotation of mobile genetic elements in prokaryotic genomes.Molecular systems biology · 2021Article
- The Role of Gram-Negative Bacteria in Urinary Tract Infections: Current Concepts and Therapeutic Options.Advances in experimental medicine and biology · 2021Review
- DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island.Nucleic acids research · 2019Article
- Pathogenesis ofEcoSal Plus · 2018Review
- Comparative Genomic Analysis of a Clinical Isolate ofFrontiers in microbiology · 2018Article
- A severe Morganella morganii endophthalmitis; followed by bacteremia.Iranian journal of microbiology · 2016Article
- Article
- Proteus mirabilis and Urinary Tract Infections.Microbiology spectrum · 2015Review
- Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis.Nature reviews. Microbiology · 2012Review
- ICEberg: a web-based resource for integrative and conjugative elements found in Bacteria.Nucleic acids research · 2012Article
- Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes.BMC genomics · 2012Article
- The phn Island: A New Genomic Island Encoding Catabolism of Polynuclear Aromatic Hydrocarbons.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Integrative and conjugative elements (ICEs), which are chromosomal mobile elements, can conjugatively transfer between bacteria. Recently, we identified a genomic island of Proteus mirabilis, a common agent of catheter-associated urinary tract infection (UTI), that possesses all the properties consistent with an ICE. This element, designated ICEPm1, is highly conserved in other causative agents of UTI, suggesting its mobility. We demonstrate that ICEPm1 can actively excise from the chromosome in a clonal population of bacteria and that this excision is integrase dependent. Although in P. mirabilis HI4320, ICEPm1 is annotated as integrated into the phenylalanine tRNA gene pheV, we show that ICEPm1 can integrate into either pheV or pheU. We determined that ICEPm1 transfers at a frequency of 1.35 × 10(-5) transconjugants/donor to ICEPm1-deficient P. mirabilis using plate mating assays with clinical isolates. Insertional inactivation of a putative integrase gene on ICEPm1 decreased transfer frequencies of ICEPm1 to below the limit of detection. Mutation of the relaxase of ICEPm1 also eliminates transfer and demonstrates that this element is indeed self-transmissible and not transferred in trans, as are some mobilizable genomic islands. Together, these findings clearly demonstrate that ICEPm1 can actively excise from the chromosome in an integrase-dependent manner, dynamically integrate into both phenylalanine tRNA genes, and transfer into clinical strains using its own conjugation machinery.
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