Evidence map›Paper›PMID 21663926›Full record

ReviewMutation research2012

Telomere length measurement-caveats and a critical assessment of the available technologies and tools.

Geraldine Aubert, Mark Hills, Peter M Lansdorp

Open access · greenAbstract readEvaluation StudyReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Mutation research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 180 papers, 6 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
180citing papers in PubMed, 6 pooled it
11.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

180 citing papers in PubMed, 6 syntheses or guidelines pooled it, 348 citations in OpenAlex.

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  5. The relationship between telomere length and mortality risk in non-model vertebrate systems: a meta-analysis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018
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120 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Geraldine AubertTerry Fox Laboratory, BC Cancer Agency, Vancouver BC, Canada.
Mark Hills
Peter M Lansdorp
BC Cancer Agency · CAUniversity of British Columbia · CA

Funding

Chromatin maintenance and sister chromatid differentiationR01GM094146 · NIGMS · BRITISH COLUMBIA CANCER AGENCY · PI LANSDORP, PETER M · 2010 to 2013
$929k
CIHR MOP38075NIGMS NIH HHS R01 GM094146PHS HHS GMH79042
6 · The paper itself

Abstract

Studies of telomeres and telomere biology often critically rely on the detection of telomeric DNA and measurements of the length of telomere repeats in either single cells or populations of cells. Several methods are available that provide this type of information and it is often not clear what method is most appropriate to address a specific research question. The major variables that need to be considered are the material that is or can be made available and the accuracy of measurements that is required. The goal of this review is to provide a comprehensive summary of the most commonly used methods and discuss the advantages and disadvantages of each. Methods that start with genomic DNA include telomere restriction fragment (TRF) length analysis, PCR amplification of telomere repeats relative to a single copy gene by Q-PCR or MMQPCR and single telomere length analysis (STELA), a PCR-based approach that accurately measures the full spectrum of telomere lengths from individual chromosomes. A different set of methods relies on fluorescent in situ hybridization (FISH) to detect telomere repeats in individual cells or chromosomes. By including essential calibration steps and appropriate controls these methods can be used to measure telomere repeat length or content in chromosomes and cells. Such methods include quantitative FISH (Q-FISH) and flow FISH which are based on digital microscopy and flow cytometry, respectively. Here the basic principles of various telomere length measurement methods are described and their strengths and weaknesses are highlighted. Some recent developments in telomere length analysis are also discussed. The information in this review should facilitate the selection of the most suitable method to address specific research question about telomeres in either model organisms or human subjects.

Indexed as

Genetic TechniquesBiotechnologyFlow CytometryHumansIn Situ Hybridization, FluorescencePolymerase Chain ReactionPolymorphism, Restriction Fragment LengthTelomereWeights and Measures

Identifiers

PMID21663926
PMCPMC3460641
OpenAlexW2014666122

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.