ArticleBiological procedures online2011
Quantitative assessment of mammary gland density in rodents using digital image analysis.
Article in Biological procedures online, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Reciprocal stabilization of CtBP and TRIM28 represses autophagy to promote metastasis.Nature structural & molecular biology · 2025Article
- Phosphotungstic acid-enhanced microcomputed tomography for quantitative visualization of mouse mammary gland morphology.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Mouse Mammary Gland Whole Mount Density Assessment across Different Morphologies Using a Bifurcated Program for Image Processing.The American journal of pathology · 2022Article
- Toward a digital analysis of environmental impacts on rodent mammary gland density during critical developmental windows.Reproductive toxicology (Elmsford, N.Y.) · 2022Article
- A Method to Pre-Screen Rat Mammary Gland Whole-Mounts Prior To RNAscope.Journal of mammary gland biology and neoplasia · 2021Article
- Comparison of the Biological Impact of UVA and UVB upon the Skin with Functional Proteomics and Immunohistochemistry.Antioxidants (Basel, Switzerland) · 2019Article
- Involution of Breast Lobules, Mammographic Breast Density and Prognosis Among Tamoxifen-Treated Estrogen Receptor-Positive Breast Cancer Patients.Journal of clinical medicine · 2019Article
- Article
- Red Raspberry Extract Protects the Skin against UVB-Induced Damage with Antioxidative and Anti-inflammatory Properties.Oxidative medicine and cellular longevity · 2019Article
- Long-Chain Omega-3 Polyunsaturated Fatty Acids Modulate Mammary Gland Composition and Inflammation.Journal of mammary gland biology and neoplasia · 2018Article
- Inherent aerobic capacity-dependent differences in breast carcinogenesis.Carcinogenesis · 2017Article
- Mammographic density: a potential monitoring biomarker for adjuvant and preventative breast cancer endocrine therapies.Oncotarget · 2017Review
- Rethinking progesterone regulation of female reproductive cyclicity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Digital histologic analysis reveals morphometric patterns of age-related involution in breast epithelium and stroma.Human pathology · 2016Article
- Application of Sholl analysis to quantify changes in growth and development in rat mammary gland whole mounts.Reproductive toxicology (Elmsford, N.Y.) · 2015Article
- In vivo microdialysis sampling of adipokines CCL2, IL-6, and leptin in the mammary fat pad of adult female rats.Molecular bioSystems · 2014Article
- A comparing study of quantitative staining techniques for retinal neovascularization in a mouse model of oxygen-induced retinopathy.International journal of ophthalmology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRodent models have been used extensively to study mammary gland development and for studies of toxicology and carcinogenesis. Mammary gland gross morphology can visualized via the excision of intact mammary gland chains following fixation and staining with carmine using a tissue preparation referred to as a whole mount. Methods are described for the automated collection of digital images from an entire mammary gland whole mount and for the interrogation of digital data using a "masking" technique available with Image-Pro® plus image analysis software (Mediacybernetics. Silver Spring, MD).
resultsParallel to mammographic analysis in humans, measurements of rodent mammary gland density were derived from area-based or volume-based algorithms and included: total circumscribed mammary fat pad mass, mammary epithelial mass, and epithelium-free fat pad mass. These values permitted estimation of absolute mass of mammary epithelium as well as breast density. The biological plausibility of these measurements was evaluated in mammary whole mounts from rats and mice. During mammary gland development, absolute epithelial mass increased linearly without significant changes in mammographic density. Treatment of rodents with tamoxifen, 9-cis-retinoic acid, or ovariectomy, and occurrence of diet induced obesity decreased both absolute epithelial mass and mammographic density. The area and volumetric methods gave similar results.
conclusionsDigital image analysis can be used for screening agents for potential impact on reproductive toxicity or carcinogenesis as well as for mechanistic studies, particularly for cumulative effects on mammary epithelial mass as well as translational studies of mechanisms that explain the relationship between epithelial mass and cancer risk.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.