ArticleBMC genomics2011
Identification of ejaculated proteins in the house mouse (Mus domesticus) via isotopic labeling.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Physiological functions of seminal vesicle secretions in male fertility.Histology and histopathology · 2026Review
- Mating system variation and gene expression in the male reproductive tract of Peromyscus mice.Molecular ecology · 2025Article
- Proteomic diversification of spermatostyles among six species of whirligig beetles.Molecular reproduction and development · 2024Article
- Serotonin neurons in mating female mice are activated by male ejaculation.Current biology : CB · 2023Article
- A Narrative Review Discussing Vasectomy-Related Impact upon the Status of Oxidative Stress and Inflammation Biomarkers and Semen Microbiota.Journal of clinical medicine · 2023Review
- Article
- Evolutionary, proteomic, and experimental investigations suggest the extracellular matrix of cumulus cells mediates fertilization outcomes†.Biology of reproduction · 2021Article
- Pronounced Postmating Response in the Drosophila Female Reproductive Tract Fluid Proteome.Molecular & cellular proteomics : MCP · 2021Article
- Social status and ejaculate composition in the house mouse.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Revealing mechanisms of mating plug function under sexual selection.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolution of Reproductive Behavior.Genetics · 2020Review
- Mating to Intact, but Not Vasectomized, Males Elicits Changes in the Endometrial Transcriptome: Insights From the Bovine Model.Frontiers in cell and developmental biology · 2020Article
- Effect of Exposure to Seminal Plasma Through Natural Mating in Cattle on Conceptus Length and Gene Expression.Frontiers in cell and developmental biology · 2020Article
- Species-specific and collection method-dependent differences in endometrial susceptibility to seminal plasma-induced RNA degradation.Scientific reports · 2019Article
- Quantitative Proteomics Identification of Seminal Fluid Proteins in MaleMolecular & cellular proteomics : MCP · 2019Article
- Specificity of the female's local cellular immune response in genital plug producing scorpion species.PloS one · 2019Article
- A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm.BMC evolutionary biology · 2018Article
- Rapid Gene Family Evolution of a Nematode Sperm Protein Despite Sequence Hyper-conservation.G3 (Bethesda, Md.) · 2018Article
- Evolutionary Dynamics of Male Reproductive Genes in theG3 (Bethesda, Md.) · 2017Article
- Multiple Genes Cause Postmating Prezygotic Reproductive Isolation in the Drosophila virilis Group.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundSeminal fluid plays an important role in successful fertilization, but knowledge of the full suite of proteins transferred from males to females during copulation is incomplete. The list of ejaculated proteins remains particularly scant in one of the best-studied mammalian systems, the house mouse (Mus domesticus), where artificial ejaculation techniques have proven inadequate. Here we investigate an alternative method for identifying ejaculated proteins, by isotopically labeling females with 15N and then mating them to unlabeled, vasectomized males. Proteins were then isolated from mated females and identified using mass spectrometry. In addition to gaining insights into possible functions and fates of ejaculated proteins, our study serves as proof of concept that isotopic labeling is a powerful means to study reproductive proteins.
resultsWe identified 69 male-derived proteins from the female reproductive tract following copulation. More than a third of all spectra detected mapped to just seven genes known to be structurally important in the formation of the copulatory plug, a hard coagulum that forms shortly after mating. Seminal fluid is significantly enriched for proteins that function in protection from oxidative stress and endopeptidase inhibition. Females, on the other hand, produce endopeptidases in response to mating. The 69 ejaculated proteins evolve significantly more rapidly than other proteins that we previously identified directly from dissection of the male reproductive tract.
conclusionOur study attempts to comprehensively identify the proteins transferred from males to females during mating, expanding the application of isotopic labeling to mammalian reproductive genomics. This technique opens the way to the targeted monitoring of the fate of ejaculated proteins as they incubate in the female reproductive tract.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.