ArticleJournal of translational medicine2011
Buffy coat specimens remain viable as a DNA source for highly multiplexed genome-wide genetic tests after long term storage.
Article in Journal of translational medicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 3 of them syntheses that pooled it.
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Who cites it
19 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Insulin and glucose-lowering agents for treating people with diabetes and chronic kidney disease.The Cochrane database of systematic reviews · 2018Pooled it
- Glucose targets for preventing diabetic kidney disease and its progression.The Cochrane database of systematic reviews · 2017Pooled it
- Association between XRCC3 Thr241Met polymorphism and nasopharyngeal carcinoma risk: evidence from a large-scale case-control study and a meta-analysis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Pooled it
- A Type 2 Diabetes Subtype Responsive to ACCORD Intensive Glycemia Treatment.Diabetes care · 2021Trial
- Flavonoid intake and telomere length attrition among middle-aged women: a cross-sectional analysis of the Nurses' Health Study.GeroScience · 2026Article
- Article
- Feasibility of extracting usable DNA from blood samples stored up to 21 years in the DiPiS study.Scientific reports · 2025Article
- Methodology for Biological Sample Collection, Processing, and Storage in the Newcastle 1000 Pregnancy Cohort: Protocol for a Longitudinal, Prospective Population-Based Study in Australia.JMIR research protocols · 2024Article
- Genetic factors help explain the variable responses of young children with cystic fibrosis to vitamin D supplements.Clinical nutrition ESPEN · 2022Article
- Preanalytical DNA assessment for downstream applications: How to optimize the management of human biospecimens to support molecular diagnosis-An experimental study.Journal of clinical laboratory analysis · 2022Article
- Factor H-related protein 1 (FHR-1) is associated with atherosclerotic cardiovascular disease.Scientific reports · 2021Article
- Paraoxonase-1 (PON1) Status Analysis Using Non-Organophosphate Substrates.Current protocols · 2021Article
- High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous atmosphere.PloS one · 2017Article
- Genetic Predictors of Cardiovascular Mortality During Intensive Glycemic Control in Type 2 Diabetes: Findings From the ACCORD Clinical Trial.Diabetes care · 2016Article
- Serum GADD45a methylation is a useful biomarker to distinguish benign vs malignant prostate disease.British journal of cancer · 2015Article
- Maximizing DNA yield for epidemiologic studies: no more buffy coats?American journal of epidemiology · 2013Article
- High-throughput real-time PCR-based genotyping without DNA purification.BMC research notes · 2012Article
- Comprehensive genomic studies: emerging regulatory, strategic, and quality assurance challenges for biorepositories.American journal of clinical pathology · 2012Review
- Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
backgroundBlood specimen collection at an early study visit is often included in observational studies or clinical trials for analysis of secondary outcome biomarkers. A common protocol is to store buffy coat specimens for future DNA isolation and these may remain in frozen storage for many years. It is uncertain if the DNA remains suitable for modern genome wide association (GWA) genotyping.
methodsWe isolated DNA from 120 Action to Control Cardiovascular Risk in Diabetes (ACCORD) clinical trial buffy coats sampling a range of storage times up to 9 years and other factors that could influence DNA yield. We performed TaqMan SNP and GWA genotyping to test whether the DNA retained integrity for high quality genetic analysis.
resultsWe tested two QIAGEN automated protocols for DNA isolation, preferring the Compromised Blood Protocol despite similar yields. We isolated DNA from all 120 specimens (yield range 1.1-312 ug per 8.5 ml ACD tube of whole blood) with only 3/120 samples yielding < 10 ug DNA. Age of participant at blood draw was negatively associated with yield (mean change -2.1 ug/year). DNA quality was very good based on gel electrophoresis QC, TaqMan genotyping of 6 SNPs (genotyping no-call rate 1.1% in 702 genotypes), and excellent quality GWA genotyping data (maximum per sample genotype missing rate 0.64%).
conclusionsWhen collected as a long term clinical trial or biobank specimen for DNA, buffy coats can be stored for up to 9 years in a -80°C frozen state and still produce high yields of DNA suitable for GWA analysis and other genetic testing.
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