ArticleThe Journal of cell biology1990
Glycolipids and transmembrane signaling: antibodies to galactocerebroside cause an influx of calcium in oligodendrocytes.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 130 citations in OpenAlex.
- Article
- Oligodendroglial membrane dynamics in relation to myelin biogenesis.Cellular and molecular life sciences : CMLS · 2016Review
- Intact lipid imaging of mouse brain samples: MALDI, nanoparticle-laser desorption ionization, and 40 keV argon cluster secondary ion mass spectrometry.Analytical and bioanalytical chemistry · 2016Article
- The Role of 3-O-Sulfogalactosylceramide, Sulfatide, in the Lateral Organization of Myelin Membrane.Neurochemical research · 2016Review
- Cross-Linking Mast Cell Specific Gangliosides Stimulates the Release of Newly Formed Lipid Mediators and Newly Synthesized Cytokines.Mediators of inflammation · 2016Article
- A case with anti-galactocerebroside antibody-positive Mycoplasma pneumoniae meningoencephalitis presenting secondary hypersomnia.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2012Article
- Ceramide galactosyltransferase (UGT8) is a molecular marker of breast cancer malignancy and lung metastases.British journal of cancer · 2010Article
- Adult CST-null mice maintain an increased number of oligodendrocytes.Journal of neuroscience research · 2009Article
- Oligodendrocyte development and myelin biogenesis: parsing out the roles of glycosphingolipids.Physiology (Bethesda, Md.) · 2009Review
- Immunostaining for oligodendrocyte-specific galactosphingolipids in fixed brain sections using the cholesterol-selective detergent digitonin.Journal of neuroscience methods · 2009Article
- Cyclic GMP-dependent pathways protect differentiated oligodendrocytes from multiple types of injury.Neurochemical research · 2007Article
- Protection of mature oligodendrocytes by inhibitors of caspases and calpains.Neurochemical research · 2003Article
- Glycosyltransferases in different brain regions during chick embryo development.Neurochemical research · 2002Article
- Structure of hydroxylated galactocerebrosides from myelin at the air-water interface.Biophysical journal · 2002Article
- Human monoclonal antibodies reactive to oligodendrocytes promote remyelination in a model of multiple sclerosis.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Cross-reactivity of anti-galactocerebroside autoantibodies with a Trypanosoma brucei proteolipidic epitope.Clinical and experimental immunology · 2000Article
- Negative regulation of oligodendrocyte differentiation by galactosphingolipids.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Targeting of IgMkappa antibodies to oligodendrocytes promotes CNS remyelination.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Tenascin-R is an intrinsic autocrine factor for oligodendrocyte differentiation and promotes cell adhesion by a sulfatide-mediated mechanism.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Release of intracellular calcium stores leads to retraction of membrane sheets and cell death in mature mouse oligodendrocytes.Neurochemical research · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This is the first study to provide evidence that one function for the surface glycolipid galactocerebroside (GalC) is participation in the opening of Ca2+ channels in oligodendroglia in culture. This glycolipid is a unique differentiation marker for myelin-producing cells; antibodies to GalC have been shown to markedly alter oligodendroglial morphology via disruption of microtubules (Dyer, C. A., and J. A. Benjamins. 1988. J. Neurosci. 8:4307-4318). This study demonstrates that extracellular EGTA blocks anti-GalC-induced disassembly of microtubules in oligodendroglial membrane sheets, demonstrating that an influx of extracellular Ca2+ mediates the cytoskeletal changes. The Ca2+ influx was examined directly by loading oligodendroglia with the fluorescent dye Indo-1 in defined medium, and measuring changes in Ca2+ in individual cells with a laser cytometer. Upon addition of anti-GalC IgG, a marked sustained increase in intracellular Ca2+ occurred in 80% of the oligodendroglia observed. EGTA blocked the increase, indicating the increase is due to an influx of extracellular Ca2+, and not due to release from intracellular stores. The effect is specific, since Ca2+ levels remain normal in oligodendroglia treated with nonimmune IgG; astrocytes do not respond to the anti-GalC. The Ca2+ response in oligodendrocytes is dependent on concentration of antibody and GalC on the oligodendroglial membrane surface. The Ca2+ influx is not mediated by voltage-sensitive Ca2+ channels: it is not blocked by cadmium, and depolarization with K+ does not mimic the response. The kinetics of the response suggest that second messenger-mediated opening of Ca2+ channels is involved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.