ArticleThe Journal of biological chemistry2011
Kinetics of doubletime kinase-dependent degradation of the Drosophila period protein.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Knockout analysis of period and timeless and EGFP-based visualization of per-expressing clock cells in the cricket circadian clock.Zoological letters · 2026Article
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- Post-Translational Modifications in Animal Circadian Clocks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Uncovering the circadian transcriptome ofProceedings. Biological sciences · 2024Article
- A circadian clock regulates the blood-brain barrier across phylogeny.Vitamins and hormones · 2024Review
- Visual and circadian regulation ofiScience · 2023Article
- Review
- Principles of the animal molecular clock learned from Neurospora.The European journal of neuroscience · 2020Review
- Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba.Scientific reports · 2019Article
- FRQ-CK1 interaction determines the period of circadian rhythms in Neurospora.Nature communications · 2019Article
- The CircadianInternational journal of molecular sciences · 2019Article
- A period without PER: understanding 24-hour rhythms without classic transcription and translation feedback loops.F1000Research · 2019Review
- Design Principles of Phosphorylation-Dependent Timekeeping in Eukaryotic Circadian Clocks.Cold Spring Harbor perspectives in biology · 2018Review
- Coordination between Differentially Regulated Circadian Clocks Generates Rhythmic Behavior.Cold Spring Harbor perspectives in biology · 2018Review
- Article
- Article
- Just-So Stories and Origin Myths: Phosphorylation and Structural Disorder in Circadian Clock Proteins.Molecular cell · 2018Article
- Making Time: Conservation of Biological Clocks from Fungi to Animals.Microbiology spectrum · 2017Review
- Molecular modulators of the circadian clock: lessons from flies and mice.Cellular and molecular life sciences : CMLS · 2017Review
- Circadian Oscillators: Around the Transcription-Translation Feedback Loop and on to Output.Trends in biochemical sciences · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Robust circadian oscillations of the proteins PERIOD (PER) and TIMELESS (TIM) are hallmarks of a functional clock in the fruit fly Drosophila melanogaster. Early morning phosphorylation of PER by the kinase Doubletime (DBT) and subsequent PER turnover is an essential step in the functioning of the Drosophila circadian clock. Here using time-lapse fluorescence microscopy we study PER stability in the presence of DBT and its short, long, arrhythmic, and inactive mutants in S2 cells. We observe robust PER degradation in a DBT allele-specific manner. With the exception of doubletime-short (DBT(S)), all mutants produce differential PER degradation profiles that show direct correspondence with their respective Drosophila behavioral phenotypes. The kinetics of PER degradation with DBT(S) in cell culture resembles that with wild-type DBT and posits that, in flies DBT(S) likely does not modulate the clock by simply affecting PER degradation kinetics. For all the other tested DBT alleles, the study provides a simple model in which the changes in Drosophila behavioral rhythms can be explained solely by changes in the rate of PER degradation.
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Registered trials
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