ArticleThe Journal of biological chemistry2011
Stimulation of yeast telomerase activity by the ever shorter telomere 3 (Est3) subunit is dependent on direct interaction with the catalytic protein Est2.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 41 citations in OpenAlex.
- Cryo-electron microscopy structure of the budding yeast telomerase holoenzyme.Science (New York, N.Y.) · 2026Article
- Orchestrating nucleic acid-protein interactions at chromosome ends: telomerase mechanisms come into focus.Nature structural & molecular biology · 2023Review
- Article
- Insights into the structure and function of Est3 from the Hansenula polymorpha telomerase.Scientific reports · 2020Article
- Fundamental mechanisms of telomerase action in yeasts and mammals: understanding telomeres and telomerase in cancer cells.Open biology · 2017Review
- Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions.Science (New York, N.Y.) · 2015Article
- Cell populations can use aneuploidy to survive telomerase insufficiency.Nature communications · 2015Article
- Proteomics of yeast telomerase identified Cdc48-Npl4-Ufd1 and Ufd4 as regulators of Est1 and telomere length.Nature communications · 2015Article
- Human telomerase: biogenesis, trafficking, recruitment, and activation.Genes & development · 2015Review
- A second essential function of the Est1-binding arm of yeast telomerase RNA.RNA (New York, N.Y.) · 2015Article
- Regulated assembly and disassembly of the yeast telomerase quaternary complex.Genes & development · 2014Article
- Identification of human TERT elements necessary for telomerase recruitment to telomeres.eLife · 2014Article
- Normal telomere length maintenance in Saccharomyces cerevisiae requires nuclear import of the ever shorter telomeres 1 (Est1) protein via the importin alpha pathway.Eukaryotic cell · 2014Article
- The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.Genetics · 2014Article
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
- A new telomerase RNA element that is critical for telomere elongation.Nucleic acids research · 2013Article
- Article
- Finding the end: recruitment of telomerase to telomeres.Nature reviews. Molecular cell biology · 2013Review
- A popular engagement at the ends.Nature structural & molecular biology · 2013Article
- Cdc13 at a crossroads of telomerase action.Frontiers in oncology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Telomerase is a multisubunit enzyme that maintains genome stability through its role in telomere replication. Although the Est3 protein is long recognized as an essential telomerase component, how it associates with and functions in the telomerase complex has remained enigmatic. Here we provide the first evidence of a direct interaction between Saccharomyces cerevisiae Est3p and the catalytic protein subunit (Est2p) by demonstrating that recombinant Est3p binds the purified telomerase essential N-terminal (TEN) domain of Est2p in vitro. Mutations in a small cluster of amino acids predicted to lie on the surface of Est3p disrupt this interaction with Est2p, reduce assembly of Est3p with telomerase in vivo, and cause telomere shortening and senescence. We also show that recombinant Est3p stimulates telomerase activity above basal levels in vitro in a manner dependent on the Est2p TEN domain interaction. Together, these results define a direct binding interaction between Est3p and Est2p and reconcile the effect of S. cerevisiae Est3p with previous experiments showing that Est3p homologs in related yeast species influence telomerase activity. Additionally, it contributes functional support to the idea that Est3p is structurally related to the mammalian shelterin protein, TPP1, which also influences telomerase activity through interaction with the Est2p homolog, TERT.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.