ArticlePlant & cell physiology2011
Plasma membrane domains participate in pH banding of Chara internodal cells.
Article in Plant & cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Optimized Laboratory Maintenance and Functional Testing of Chara braunii.Current protocols · 2026Article
- Evidence for a COThe New phytologist · 2025Article
- The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii.The New phytologist · 2025Article
- Origin and evolution of auxin-mediated acid growth.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Re-Establishment Techniques and Transplantations of Charophytes to Support Threatened Species.Plants (Basel, Switzerland) · 2021Review
- PH-dependent cell-cell interactions in the green alga Chara.Protoplasma · 2019Article
- Article
- High efficiency and long-term intracellular activity of an enzymatic nanofactory based on metal-organic frameworks.Nature communications · 2017Article
- Clathrin inFrontiers in plant science · 2017Article
- Is Wortmannin-Induced Reorganization of the trans-Golgi Network the Key to Explain Charasome Formation?Frontiers in plant science · 2016Article
- Convoluted Plasma Membrane Domains in the Green Alga Chara are Depleted of Microtubules and Actin Filaments.Plant & cell physiology · 2015Article
- Photosynthesis-dependent formation of convoluted plasma membrane domains in Chara internodal cells is independent of chloroplast position.Protoplasma · 2015Article
- Molecular Analysis and Localization of CaARA7 a Conventional RAB5 GTPase from Characean Algae.Traffic (Copenhagen, Denmark) · 2015Article
- Cellular Auxin Transport in Algae.Plants (Basel, Switzerland) · 2014Review
- Heterotrimeric G proteins in green algae: an early innovation in the evolution of the plant lineage.Plant signaling & behavior · 2014Article
- Vesicular trafficking in characean green algae and the possible involvement of a VAMP72-family protein.Plant signaling & behavior · 2014Article
- Characterization of the heterotrimeric G-protein complex and its regulator from the green alga Chara braunii expands the evolutionary breadth of plant G-protein signaling.Plant physiology · 2013Article
- Molecular and biochemical analysis of the first ARA6 homologue, a RAB5 GTPase, from green algae.Journal of experimental botany · 2013Article
- Further electrophysiological studies on cellular effect of herbicide, bromoxynil, using characean cells.Journal of plant research · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We investigated the identity and distribution of cortical domains, stained by the endocytic marker FM 1-43, in branchlet internodal cells of the characean green algae Chara corallina and Chara braunii. Co-labeling with NBD C(6)-sphingomyelin, a plasma membrane dye, which is not internalized, confirmed their location in the plasma membrane, and co-labelling with the fluorescent pH indicator Lysotracker red indicated an acidic environment. The plasma membrane domains co-localized with the distribution of an antibody against a proton-translocating ATPase, and electron microscopic data confirmed their identity with elaborate plasma membrane invaginations known as charasomes. The average size and the distribution pattern of charasomes correlated with the pH banding pattern of the cell. Charasomes were larger and more frequent at the acidic regions than at the alkaline bands, indicating that they are involved in outward-directed proton transport. Inhibition of photosynthesis by DCMU prevented charasome formation, and incubation in pH buffers resulted in smaller, homogenously distributed charasomes irrespective of whether the pH was clamped at 5.5 or 8.5. These data indicate that the differential size and distribution of charasomes is not due to differences in external pH but reflects active, photosynthesis-dependent pH banding. The fact that pH banding recovered within several minutes in unbuffered medium, however, confirms that pH banding is also possible in cells with evenly distributed charasomes or without charasomes. Cortical mitochondria were also larger and more abundant at the acid bands, and their intimate association with charasomes and chloroplasts suggests an involvement in carbon uptake and photorespiration.
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