ArticleLangmuir : the ACS journal of surfaces and colloids2011
Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation.
Article in Langmuir : the ACS journal of surfaces and colloids, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 55 citations in OpenAlex.
- A Synergistic Overview between Microfluidics and Numerical Research for Vascular Flow and Pathological Investigations.Sensors (Basel, Switzerland) · 2024Review
- Effect of upstream priming on transient downstream platelet-substrate interactions.Colloids and surfaces. B, Biointerfaces · 2021Article
- Downstream platelet adhesion and activation under highly elevated upstream shear forces.Acta biomaterialia · 2019Article
- Effects of upstream shear forces on priming of platelets for downstream adhesion and activation.Acta biomaterialia · 2018Article
- Binary agonist surface patterns prime platelets for downstream adhesion in flowing whole blood.Biointerphases · 2017Article
- RGDfK-functionalized gold nanorods bind only to activated platelets.Journal of biomedical materials research. Part A · 2017Article
- Functional assay of antiplatelet drugs based on margination of platelets in flowing blood.Biointerphases · 2016Article
- Surface modification on polydimethylsiloxane-based microchannels with fragmented poly(l-lactic acid) nanosheets.Biomicrofluidics · 2015Article
- Platelet geometry sensing spatially regulates α-granule secretion to enable matrix self-deposition.Blood · 2015Article
- The platelet and the biophysical microenvironment: lessons from cellular mechanics.Thrombosis research · 2014Review
- Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans.Acta biomaterialia · 2013Article
- Microfluidics and coagulation biology.Annual review of biomedical engineering · 2013Review
- The role of fibrinogen spacing and patch size on platelet adhesion under flow.Acta biomaterialia · 2012Article
- The effect of upstream platelet-fibrinogen interactions on downstream adhesion and activation.Biomaterials · 2012Article
- Multiprotein microcontact printing with micrometer resolution.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Microenvironmental geometry guides platelet adhesion and spreading: a quantitative analysis at the single cell level.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to promote or inhibit specific platelet-surface interactions in well-controlled environments is crucial to studying fundamental adhesion and activation mechanisms. Here, microcontact printing was used to immobilize human fibrinogen covalently in the form of randomly placed, micrometer-sized islands at an overall surface coverage of 20, 50, or 85%. The nonprinted background region was blocked with covalently immobilized human albumin. Platelet adhesion and morphology on each substrate were assessed using combined differential interference and fluorescence microscopy. At 20% coverage, most of the fibrinogen surface features were small round islands, and platelet adhesion and spreading areas were limited by the position and the size of the islands. Platelet circularity, indicated the morphology was mostly rounded. At 50% coverage, some fibrinogen islands coalesced and platelet adhesion and spreading areas increased. Platelet morphology was controlled by the shape of underlying fibrinogen islands, leading to more irregular spreading. At 85% coverage, the fibrinogen pattern was completely interconnected and both platelet adhesion and the spreading area were significantly higher than at lower coverage. In addition, platelets also spread over the albumin regions, suggesting that after a critical surface density of fibrinogen ligands is reached, platelet spreading is no longer inhibited by albumin. Increasing the overall fibrinogen coverage resulted in higher activation levels defined by key morphological characteristics of the spreading platelet.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.