ArticleProteomics. Clinical applications2011
Utility of formalin-fixed, paraffin-embedded liver biopsy specimens for global proteomic analysis in nonalcoholic steatohepatitis.
Article in Proteomics. Clinical applications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Proteomics identifies complement protein signatures in patients with alcohol-associated hepatitis.JCI insight · 2024Article
- Proteomic profiling of formalin-fixed paraffine-embedded tissue reveals key proteins related to lung dysfunction in idiopathic pulmonary fibrosis.Frontiers in oncology · 2023Article
- Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi.Current issues in molecular biology · 2022Article
- Lyophilized human cells stored at room temperature preserve multiple RNA species at excellent quality for RNA sequencing.Oncotarget · 2018Article
- Fast and Simple Protocols for Mass Spectrometry-Based Proteomics of Small Fresh Frozen Uterine Tissue Sections.Analytical chemistry · 2017Article
- Proteomic analysis of neurons microdissected from formalin-fixed, paraffin-embedded Alzheimer's disease brain tissue.Scientific reports · 2015Article
- Nonalcoholic steatohepatitis in precision medicine: Unraveling the factors that contribute to individual variability.Pharmacology & therapeutics · 2015Review
- Tissue proteomics in pancreatic cancer study: discovery, emerging technologies, and challenges.Proteomics · 2013Review
- Selected Reaction Monitoring (SRM) Analysis of Epidermal Growth Factor Receptor (EGFR) in Formalin Fixed Tumor Tissue.Clinical proteomics · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
purposeTo compare the proteomic profiles of formalin-fixed, paraffin-embedded (FFPE) liver biopsy material and matched frozen liver tissue from patients with nonalcoholic steatohepatitis (NASH). EXPERIMENTAL
designA label-free mass spectrometry-based approach was used to profile global protein expression in FFPE and frozen liver biopsy specimens from five patients with NASH.
resultsEight hundred and sixty proteins were identified with >75% confidence: 225 common proteins were identified in both the FFPE and frozen tissues, and an additional 142 and 493 proteins were identified in the FFPE and frozen tissues, respectively. Functional analyses revealed a general, nonspecific reduction in the number of proteins identified in FFPE tissue compared with frozen tissue. No bias toward proteins located in any specific subcellular compartments or implicated in any particular biological functions was observed. The relative abundance of several proteins with functions relating to the pathogenesis of NASH (peroxiredoxin-1, fatty acid binding protein 1, fatty acid synthase, vimentin, catalase, and glutathione S-transferase A1) was similar in FFPE and frozen liver tissues. CONCLUSIONS AND CLINICAL RELEVANCE: FFPE liver biopsy material from NASH patients can be used for global proteomic analysis and biomarker identification studies, although a universal reduction in the number of identified proteins compared with frozen tissue is likely.
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