Evidence map›Paper›PMID 21656745›Full record

ArticleCancer2011

Tristetraprolin regulates interleukin-6, which is correlated with tumor progression in patients with head and neck squamous cell carcinoma.

Elizabeth Van Tubergen, Robert Vander Broek, Julia Lee, Gregory Wolf, Thomas Carey, Carol Bradford, Mark Prince, Keith L Kirkwood, Nisha J D'Silva

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 67 citations in OpenAlex.

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  11. The mRNA-destabilizing protein Tristetraprolin targets "meiosis arrester"Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
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  15. Tristetraprolin Regulates TFrontiers in immunology · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Elizabeth Van TubergenDepartment of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109-1078, USA.
Robert Vander Broek
Julia Lee
Gregory Wolf
Thomas Carey
Carol Bradford
Mark Prince
Keith L Kirkwood
Nisha J D'Silva
University of Michigan–Ann Arbor · USMedical University of South Carolina · US

Funding

Therapeutic Potential and Mechanism of Action of Potent Small Molecule InhibitorsP50CA097248 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WOLF, GREGORY T · 2002 to 2013
$25.3M
THE ROLE OF THE RNA BINDING PROTEIN LA IN ORAL TUMOR BIOLOGYP20RR017696 · NCRR · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI KIRKWOOD, KEITH L · 2002 to 2011
$20.7M
Tissue Engineering and RegenerationT32DE007057 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DAVID H. KOHN · 1985 to 2026
$17.3M
Post-Transcriptional Regulation of Periodontal DiseR01DE021423 · NIDCR · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI KIRKWOOD, KEITH L · 2011 to 2015
$2.1M
Negative Regulation of Innate Immune Responses through MAPK PhosphatasesR01DE018290 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KIRKWOOD, KEITH L · 2007 to 2011
$1.8M
Role of Rap1 in cadherin and integrin mediated cross-talk in oral keratinocytesR01DE018512 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI D'SILVA, NISHA J · 2007 to 2011
$1.7M
Biomarkers in Head and Neck Cancer ProgressionK02DE019513 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI D'SILVA, NISHA J · 2009 to 2013
$472k
Characterization of the Immunomic Profile of Head and Neck CancerR21DE017977 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI D'SILVA, NISHA J · 2008 to 2009
$418k
Silencing Post Transcriptional Signaling Mechanisms in Periodontal InflammationR21DE019272 · NIDCR · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI KIRKWOOD, KEITH L · 2008 to 2009
$406k
Altering Cytokine mRNA Stability in Periodontal Bone LossR21DE017966 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KIRKWOOD, KEITH L · 2007 to 2008
$393k
The role of tristetraprolin in head and neck cancer progressionF32DE021305 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI VAN TUBERGEN, ELIZABETH ANNE · 2010 to 2012
$142k
NCI NIH HHS P50 CA097248NCI NIH HHS P50-CA97248NCRR NIH HHS P20 RR017696NIDCR NIH HHS 5F32 DE213052NIDCR NIH HHS 5R21 DE017966NIDCR NIH HHS F32 DE021305NIDCR NIH HHS K02 DE019513NIDCR NIH HHS K02-DE019513NIDCR NIH HHS R01 DE018290NIDCR NIH HHS R01-DE018290NIDCR NIH HHS R01 DE018512NIDCR NIH HHS R01-DE018512NIDCR NIH HHS R01 DE021423NIDCR NIH HHS R21 DE017966NIDCR NIH HHS R21 DE017977NIDCR NIH HHS R21-DE017977NIDCR NIH HHS R21 DE019272NIDCR NIH HHS T32 DE007057NIDCR NIH HHS T32-DE007057-33
6 · The paper itself

Abstract

backgroundTumor-derived cytokines play a significant role in the progression of head and neck squamous cell carcinoma (HNSCC). Targeting proteins, such as tristetraprolin (TTP), that regulate multiple inflammatory cytokines may inhibit the progression of HNSCC. However, TTP's role in cancer is poorly understood. The goal of the current study was to determine whether TTP regulates inflammatory cytokines in patients with HNSCC.

methodsTTP messenger RNA (mRNA) and protein expression were determined by quantitative real-time-polymerase chain reaction (Q-RT-PCR) and Western blot analysis, respectively. mRNA stability and cytokine secretion were evaluated by quantitative RT-PCR and enzyme-linked immunoadsorbent assay, respectively, after overexpression or knockdown of TTP in HNSCC. HNSCC tissue microarrays were immunostained for interleukin-6 (IL-6) and TTP.

resultsTTP expression in HNSCC cell lines was found to be inversely correlated with the secretion of IL-6, vascular endothelial growth factor (VEGF), and prostaglandin E2 (PGE(2) )(.) Knockdown of TTP increased mRNA stability and the secretion of cytokines. Conversely, overexpression of TTP in HNSCC cells led to decreased secretion of IL-6, VEGF, and PGE(2) . Immunohistochemical staining of tissue microarrays for IL-6 demonstrated that staining intensity is prognostic for poor disease-specific survival (P = .023), tumor recurrence and development of second primary tumors (P = .014), and poor overall survival (P = .019).

conclusionsThe results of the current study demonstrated that down-regulation of TTP in HNSCC enhances mRNA stability and promotes secretion of IL-6, VEGF, and PGE(2) . Furthermore, high IL-6 secretion in HNSCC tissue is a biomarker for poor prognosis. In as much as enhanced cytokine secretion is associated with poor prognosis, TTP may be a therapeutic target to reduce multiple cytokines concurrently in patients with HNSCC.

Indexed as

CarcinomaCarcinoma, Squamous CellCell Line, TumorCell MovementDisease ProgressionHead and Neck NeoplasmsHumansInterleukin-6Neoplasm InvasivenessNeoplasms, Squamous Cellp38 Mitogen-Activated Protein KinasesRNA StabilitySquamous Cell Carcinoma of Head and NeckTristetraprolinVascular Endothelial Growth Factor AInterleukin-6p38 Mitogen-Activated Protein KinasesTristetraprolinVascular Endothelial Growth Factor A

Identifiers

PMID21656745
PMCPMC3574798
OpenAlexW1599962373

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.