ArticlePloS one2011
The essential functions of NEDD8 are mediated via distinct surface regions, and not by polyneddylation in Schizosaccharomyces pombe.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Protein neddylation and its role in health and diseases.Signal transduction and targeted therapy · 2024Review
- Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The necessity of NEDD8/Rub1 for vitality and its association with mitochondria-derived oxidative stress.Redox biology · 2020Review
- A high throughput mutagenic analysis of yeast sumo structure and function.PLoS genetics · 2017Article
- Ubiquitylation, neddylation and the DNA damage response.Open biology · 2015Review
- Protein neddylation: beyond cullin-RING ligases.Nature reviews. Molecular cell biology · 2015Review
- The NEDD8 modification pathway in plants.Frontiers in plant science · 2014Review
- Phage display to identify Nedd8-mimicking peptides as inhibitors of the Nedd8 transfer cascade.Chembiochem : a European journal of chemical biology · 2013Article
- Nedd8 processing enzymes in Schizosaccharomyces pombe.BMC biochemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
The ubiquitin-like protein NEDD8 is highly conserved in eukaryotes, from man to Schizosaccharomyces pombe. NEDD8 conjugation to cullin proteins is a prerequisite for cullin based E3 ubiquitin ligase activity, and essential for S. pombe viability. Here, we have performed alanine scanning mutagenesis of all conserved surface residues and show that the majority of essential residues were located around the hydrophobic patch and the C-terminus. However, we further identified essential residues not previously reported to be involved in ubiquitin ligase regulation that importantly do not prevent Ned8p conjugation. We also find that mutation of all conserved lysine residues in Ned8p, did not affect yeast viability, suggesting that mono-neddylation is sufficient for yeast viability under most conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.