ArticlePloS one2011
Taming membranes: functional immobilization of biological membranes in hydrogels.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Synthetic cells for phage therapy: a perspective.Frontiers in cellular and infection microbiology · 2025Review
- Role of adhesion forces in mechanosensitive channel gating in Staphylococcus aureus adhering to surfaces.NPJ biofilms and microbiomes · 2020Article
- A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation.Scientific reports · 2018Article
- Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells.Scientific reports · 2017Article
- Membrane protein reconstitution into giant unilamellar vesicles: a review on current techniques.European biophysics journal : EBJ · 2017Review
- Posing for a picture: vesicle immobilization in agarose gel.Scientific reports · 2016Article
- 2D and 3D self-assembling nanofiber hydrogels for cardiomyocyte culture.BioMed research international · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule studies on membrane proteins embedded in their native environment are hampered by the intrinsic difficulty of immobilizing elastic and sensitive biological membranes without interfering with protein activity. Here, we present hydrogels composed of nano-scaled fibers as a generally applicable tool to immobilize biological membrane vesicles of various size and lipid composition. Importantly, membrane proteins immobilized in the hydrogel as well as soluble proteins are fully active. The triggered opening of the mechanosensitive channel of large conductance (MscL) reconstituted in giant unilamellar vesicles (GUVs) was followed in time on single GUVs. Thus, kinetic studies of vectorial transport processes across biological membranes can be assessed on single, hydrogel immobilized, GUVs. Furthermore, protein translocation activity by the membrane embedded protein conducting channel of bacteria, SecYEG, in association with the soluble motor protein SecA was quantitatively assessed in bulk and at the single vesicle level in the hydrogel. This technique provides a new way to investigate membrane proteins in their native environment at the single molecule level by means of fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.