ArticleThe ISME journal2011
A novel fungal family of oligopeptide transporters identified by functional metatranscriptomics of soil eukaryotes.
Article in The ISME journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 84 citations in OpenAlex.
- Fungi to the rescue: recent advances, mechanistic insights and omics-based perspectives in heavy metal mycoremediation.Archives of microbiology · 2026Review
- Genomic tales of wine yeasts: from domestication to functional innovation.FEMS yeast research · 2026Review
- Biocontainment efficacy redefined.Applied and environmental microbiology · 2025Article
- Wine Fermentation as a Model System for Microbial Ecology and Evolution.Environmental microbiology · 2025Review
- Engineering the secretome of Aspergillus niger for cellooligosaccharides production from plant biomass.Microbial cell factories · 2024Article
- Metagenomics and metatranscriptomics as potential driving forces for the exploration of diversity and functions of micro-eukaryotes in soil.3 Biotech · 2023Review
- Unlocking the secrets of peptide transport in wine yeast: insights into oligopeptide transporter functions and nitrogen source preferences.Applied and environmental microbiology · 2023Article
- Genomic Adaptations ofMicroorganisms · 2022Review
- Yeast Plasma Membrane Fungal Oligopeptide Transporters Display Distinct Substrate Preferences despite Their High Sequence Identity.Journal of fungi (Basel, Switzerland) · 2021Article
- Metatranscriptomic analysis reveals active microbes and genes responded to short-term Cr(VI) stress.Ecotoxicology (London, England) · 2021Article
- Protection from metal toxicity by Hsp40-like protein isolated from contaminated soil using functional metagenomic approach.Environmental science and pollution research international · 2021Article
- Multi-metal tolerance of DHHC palmitoyl transferase-like protein isolated from metal contaminated soil.Ecotoxicology (London, England) · 2021Article
- New insights into HcPTR2A and HcPTR2B, two high-affinity peptide transporters from the ectomycorrhizal model fungus Hebeloma cylindrosporum.Mycorrhiza · 2020Article
- Metatranscriptomics: an approach for retrieving novel eukaryotic genes from polluted and related environments.3 Biotech · 2020Review
- Diversity of Oligopeptide Transport in Yeast and Its Impact on Adaptation to Winemaking Conditions.Frontiers in genetics · 2020Review
- Regulation of Amino Acid Transport in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2019Review
- The art of vector engineering: towards the construction of next-generation genetic tools.Microbial biotechnology · 2019Review
- Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter.Microbial cell factories · 2018Article
- Recent Insights on Biological and Ecological Aspects of Ectomycorrhizal Fungi and Their Interactions.Frontiers in microbiology · 2018Review
- Capturing the genetic makeup of the active microbiome in situ.The ISME journal · 2017Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional environmental genomics has the potential to identify novel biological functions that the systematic sequencing of microbial genomes or environmental DNA may fail to uncover. We targeted the functions expressed by soil eukaryotes using a metatranscriptomic approach based on the use of soil-extracted polyadenylated messenger RNA to construct environmental complementary DNA expression libraries. Functional complementation of a yeast mutant defective in di/tripeptide uptake identified a novel family of oligopeptide transporters expressed by fungi. This family has a patchy distribution in the Basidiomycota and Ascomycota and is present in the genome of a Saccharomyces cerevisiae wine strain. High throughput phenotyping of yeast mutants expressing two environmental transporters showed that they both displayed broad substrate specificity and could transport more than 60-80 dipeptides. When expressed in Xenopus oocytes one environmental transporter induced currents upon dipeptide addition, suggesting proton-coupled co-transport of dipeptides. This transporter was also able to transport specifically cysteine. Deletion of the two copies of the corresponding gene family members in the genome of the wine yeast strain severely reduced the number of dipeptides that it could assimilate. These results demonstrate that these genes are functional and can be used by fungi to efficiently scavenge the numerous, low concentration, oligopeptides continuously generated in soils by proteolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.