ArticleJournal of visualized experiments : JoVE2011
Generation of neural stem cells from discarded human fetal cortical tissue.
Article in Journal of visualized experiments : JoVE, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Consequences of trisomy 21 for brain development in Down syndrome.Nature reviews. Neuroscience · 2024Review
- Single-cell RNA-sequencing identifies various proportions of excitatory and inhibitory neurons in cultured human fetal brain cortical tissues.Frontiers in neuroscience · 2023Article
- Cell models for Down syndrome-Alzheimer's disease research.Neuronal signaling · 2022Review
- Modeling HIV-1 neuropathogenesis using three-dimensional human brain organoids (hBORGs) with HIV-1 infected microglia.Scientific reports · 2020Article
- Article
- Formin 2 Regulates Lysosomal Degradation of Wnt-Associated β-Catenin in Neural Progenitors.Cerebral cortex (New York, N.Y. : 1991) · 2019Article
- Isolation and culture of porcine primary fetal progenitors and neurons from the developing dorsal telencephalon.Journal of veterinary science · 2019Article
- All Together Now: Modeling the Interaction of Neural With Non-neural Systems Using Organoid Models.Frontiers in neuroscience · 2019Review
- Human Models Are Needed for Studying Human Neurodevelopmental Disorders.American journal of human genetics · 2018Review
- Filamin A- and formin 2-dependent endocytosis regulates proliferation via the canonical Wnt pathway.Development (Cambridge, England) · 2016Article
- Global hypermethylation in fetal cortex of Down syndrome due to DNMT3L overexpression.Human molecular genetics · 2016Article
- Identification and dynamic regulation of tight junction protein expression in human neural stem cells.Stem cells and development · 2015Article
- Modeling neurodevelopmental disorders using human pluripotent stem cells.Stem cell reviews and reports · 2014Review
- Gene expression changes in the MAPK pathway in both Fragile X and Down syndrome human neural progenitor cells.American journal of stem cells · 2012Article
- S100B and APP promote a gliocentric shift and impaired neurogenesis in Down syndrome neural progenitors.PloS one · 2011Article
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Authors and funding
5 authors.
Funding
Abstract
Neural stem cells (NSCs) reside along the ventricular zone neuroepithelium during the development of the cortical plate. These early progenitors ultimately give rise to intermediate progenitors and later, the various neuronal and glial cell subtypes that form the cerebral cortex. The capacity to generate and expand human NSCs (so called neurospheres) from discarded normal fetal tissue provides a means with which to directly study the functional aspects of normal human NSC development. This approach can also be directed toward the generation of NSCs from known neurological disorders, thereby affording the opportunity to identify disease processes that alter progenitor proliferation, migration and differentiation. We have focused on identifying pathological mechanisms in human Down syndrome NSCs that might contribute to the accelerated Alzheimer's disease phenotype. Neither in vivo nor in vitro mouse models can replicate the identical repertoire of genes located on human chromosome 21. Here we use a simple and reliable method to isolate Down syndrome NSCs from aborted human fetal cortices and grow them in culture. The methodology provides specific aspects of harvesting the tissue, dissection with limited anatomical landmarks, cell sorting, plating and passaging of human NSCs. We also provide some basic protocols for inducing differentiation of human NSCs into more selective cell subtypes.
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Registered trials
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