ArticleJournal of biosciences2011
Detrimental effect of expression of Bt endotoxin Cry1Ac on in vitro regeneration, in vivo growth and development of tobacco and cotton transgenics.
Article in Journal of biosciences, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Transcriptome sequencing and weighted correlation network analysis reveal the molecular mechanism of growth variation in highly resistant transgenic double Bt poplar.BMC plant biology · 2026Article
- Achieving High Expression of Cry in Green Tissues and Negligible Expression in Endosperm Simultaneously viaInternational journal of molecular sciences · 2023Article
- Individual and combined effects of land use and weeds on Cry1Ab/c protein expression and yield of transgenicGM crops & food · 2022Article
- Discovery and functional characterization of novel cotton promoters with potential application to pest control.Plant cell reports · 2022Article
- Fitness of Insect-resistant transgenic rice T1C-19 under four growing conditions combining land use and weed competition.GM crops & food · 2021Article
- Cellular Localization of Exogenous Cry1Ab/c and its Interaction with Plasma Membrane CaFrontiers in bioengineering and biotechnology · 2021Article
- Using Combined Methods of Genetic Mapping and Nanopore-Based Sequencing Technology to Analyze the Insertion Positions ofFrontiers in plant science · 2021Article
- Seed-specific activity of the Arabidopsis β-glucosidase 19 promoter in transgenic Arabidopsis and tobacco.Plant cell reports · 2021Article
- Enhanced yield performance of transgenicGM crops & food · 2020Article
- A strong early acting wound-inducible promoter, RbPCD1pro, activates cryIAc expression within minutes of wounding to impart efficient protection against insects.Plant biotechnology journal · 2019Article
- Transgenic rice plants expressing synthetic cry2AX1 gene exhibits resistance to rice leaffolder (Cnaphalocrosis medinalis).3 Biotech · 2016Article
- Development of leaffolder resistant transgenic rice expressing cry2AX1 gene driven by green tissue-specific rbcS promoter.World journal of microbiology & biotechnology · 2016Article
- Article
- Chloroplast localization of Cry1Ac and Cry2A protein--an alternative way of insect control in cotton.Biological research · 2015Article
- Comparative performance of modified full-length and truncated Bacillus thuringiensis-cry1Ac genes in transgenic tomato.SpringerPlus · 2015Article
- Transgenic tomato line expressing modified Bacillus thuringiensis cry1Ab gene showing complete resistance to two lepidopteran pests.SpringerPlus · 2014Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High levels of expression of the cry1Ac gene from Bacillus thuringiensis cannot be routinely achieved in transgenic plants despite modifications made in the gene to improve its expression. This has been attributed to the instability of the transcript in a few reports. In the present study, based on the genetic transformation of cotton and tobacco, we show that the expression of the Cry1Ac endotoxin has detrimental effects on both the in vitro and in vivo growth and development of transgenic plants. A number of experiments on developing transgenics in cotton with different versions of cry1Ac gene showed that the majority of the plants did not express any Cry1Ac protein. Based on Southern blot analysis, it was also observed that a substantial number of lines did not contain the cry1Ac gene cassette although they contained the marker gene nptII. More significantly, all the lines that showed appreciable levels of expression were found to be phenotypically abnormal. Experiments on transformation of tobacco with different constructs expressing the cry1Ac gene showed that in vitro regeneration was inhibited by the encoded protein. Further, out of a total of 145 independent events generated with the different cry1Ac gene constructs in tobacco, only 21 showed expression of the Cry1Ac protein, confirming observations made in cotton that regenerants that express high levels of the Cry1Ac protein are selected against during regeneration of transformed events. This problem was circumvented by targeting the Cry1Ac protein to the chloroplast, which also significantly improved the expression of the protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.