ArticleBiology of reproduction2011
Stage-specific changes in GDNF expression by rat Sertoli cells: a possible regulator of the replication and differentiation of stem spermatogonia.
Article in Biology of reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 82 citations in OpenAlex.
- Unveiling novel insights into gene expression in monkey testes through single-nucleus-based analysis.Frontiers in cell and developmental biology · 2026Article
- Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120.Cell proliferation · 2024Article
- Effects of Follicle-Stimulating Hormone on Human Sperm Motility In Vitro.International journal of molecular sciences · 2023Article
- Impact of germ cell ablation on the activation of the brain-pituitary-gonadal axis in precocious Atlantic salmon (Salmo salar L.) males.Molecular reproduction and development · 2022Article
- Regulation of spermatogonial stem cell self-renewal and proliferation in mammals.Histology and histopathology · 2022Review
- Sertoli Cell-Germ Cell Interactions Within the Niche: Paracrine and Juxtacrine Molecular Communications.Frontiers in endocrinology · 2022Review
- The Regulation of Spermatogonial Stem Cells in an Adult Testis by Glial Cell Line-Derived Neurotrophic Factor.Frontiers in endocrinology · 2022Review
- Spermatogonial stem cell transplantation into nonablated mouse recipient testes.Stem cell reports · 2021Article
- FSH regulates RA signaling to commit spermatogonia into differentiation pathway and meiosis.Reproductive biology and endocrinology : RB&E · 2021Review
- Androgen Actions in the Testis and the Regulation of Spermatogenesis.Advances in experimental medicine and biology · 2021Review
- BRG1 Is Dispensable for Sertoli Cell Development and Functions in Mice.International journal of molecular sciences · 2020Article
- The Sertoli cell: what can we learn from different vertebrate models?Animal reproduction · 2020Article
- Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF.eLife · 2019Article
- Regulation of GDNF expression in Sertoli cells.Reproduction (Cambridge, England) · 2019Review
- Aberrant gene expression by Sertoli cells in infertile men with Sertoli cell-only syndrome.PloS one · 2019Article
- Testicular endothelial cells are a critical population in the germline stem cell niche.Nature communications · 2018Article
- 50 years of spermatogenesis: Sertoli cells and their interactions with germ cells.Biology of reproduction · 2018Review
- Sertoli Cells Avert Neuroinflammation-Induced Cell Death and Improve Motor Function and Striatal Atrophy in Rat Model of Huntington Disease.Journal of molecular neuroscience : MN · 2018Article
- Cyclical expression of GDNF is required for spermatogonial stem cell homeostasis.Development (Cambridge, England) · 2018Article
- Evaluating the behavior of cultured sertoli cells in the presence and absence of spermatogonial stem cell.Stem cell investigation · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In the adult testis, the precise control of the self-renewing replication and differentiation of stem spermatogonia is fundamental to male fertility. Previous studies have shown that the replication of A single (A(s)) spermatogonia, a population that includes the stem cells, is maximal at stage I of the cycle of the rat seminiferous epithelium and minimal at stage VII, while the ratio of A-paired spermatogonia to A(s) spermatogonia increases from stages I to VII. It has been hypothesized that these changes in A(s) spermatogonia replication and differentiation result from changes in the expression of glial cell-line derived neurotrophic factor (GDNF) by Sertoli cells. To directly test this hypothesis, we used immunocytochemistry and confocal microscopy to demonstrate that within intact seminiferous tubules, GDNF is detectable only in Sertoli cells and that its amount and its location within these cells changes with progression of the stages of the cycle. The identification of Sertoli cells as the primary source of GDNF was confirmed by RT-PCR analysis of RNA isolated from purified populations of Sertoli cells, pachytene spermatocytes, and round spermatids. Stage-specific changes in GDNF expression were confirmed by quantifying GDNF mRNA in seminiferous tubules at defined stages of the cycle. Expression of this transcript was maximal at stage I, fell 14-fold by stage VIIc,d, and then increased 12-fold by stages XIII-XIV. This pattern of expression was the opposite of the control, cathepsin L mRNA. Taken together, these data support the hypothesis that cyclical changes in GDNF expression by Sertoli cells are responsible for the stage-specific replication and differentiation of stem spermatogonia, the foundational cells of spermatogenesis.
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