ArticleJournal of clinical microbiology2011
Detection of AmpC beta-lactamase in Escherichia coli: comparison of three phenotypic confirmation assays and genetic analysis.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed.
- Non canonical perspectives on AmpR mediated ampC expression in Gram negative bacteria, with particular emphasis on the potential regulatory purview of FtsZ.World journal of microbiology & biotechnology · 2026Review
- Antimicrobial Resistance of Serratia marcescens Recovered From Aquatic Environments: A Review.Environmental microbiology reports · 2026Review
- Article
- Integrated Phenotypic and Genomic Characterization of Cefotaxime/Clavulanic Acid Inhibitor-Positive Multidrug-ResistantAnimals : an open access journal from MDPI · 2026Article
- In Vitro Activity of Cefaclor/Beta-Lactamases Inhibitors (Clavulanic Acid and Sulbactam) Combination Against Extended-Spectrum Beta-Lactamase Producing UropathogenicAntibiotics (Basel, Switzerland) · 2025Article
- Phenotypic susceptibility profiles of AmpC- and/or extended-spectrum beta-lactamase-(co)producingJAC-antimicrobial resistance · 2025Article
- Characterization ofAntibiotics (Basel, Switzerland) · 2025Article
- Phenotypic and Molecular Characterization of Extended-Spectrum β-Lactamase, Plasmid-Mediated- AmpC, and Carbapenemase-Producing Enterobacteriaceae Isolated from Companion and Production Animals in Brazil.Current microbiology · 2025Article
- Extended spectrum, AmpC & metallo β-lactamases producing Escherichia coli in urinary isolates: A prospective study in north India.The Indian journal of medical research · 2025Article
- Direct detection of Gram-negative bacilli and extended-spectrum β-lactamase producers in positive blood culture specimens using MALDI-TOF MS.Frontiers in cellular and infection microbiology · 2025Article
- Longitudinal study on the genetic diversity of extended-spectrum cephalosporin resistantFrontiers in microbiology · 2025Article
- Fecal Carriage and Risk Factors Associated with Extended-Spectrum β-Lactamase-/AmpC-/Carbapenemase-ProducingAnimals : an open access journal from MDPI · 2024Article
- Innovative approaches in phenotypic beta-lactamase detection for personalised infection management.Nature communications · 2024Review
- Antibiotic Treatment of Infections Caused by AmpC-Producing Enterobacterales.Pharmacy (Basel, Switzerland) · 2024Review
- Host species shapes genotype, antimicrobial resistance, and virulence profiles of enterotoxigenicApplied and environmental microbiology · 2024Article
- Performance of Flow Cytometry-Based Rapid Assay in Detection of Carbapenemase-Producing Enterobacterales.International journal of molecular sciences · 2024Article
- Beta-lactamase dependent and independent evolutionary paths to high-level ampicillin resistance.Nature communications · 2024Article
- Global epistasis in plasmid-mediated antimicrobial resistance.Molecular systems biology · 2024Review
- Genomic characterization of extended-spectrum beta-lactamase-producing and carbapenem-resistant Escherichia coli from urban wastewater in Australia.MicrobiologyOpen · 2024Article
- Rare Plasmid-Mediated AmpC Beta-Lactamase DHA-1 Located on Easy Mobilized IS26-Related Genetic Element Detected inMicroorganisms · 2024Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two mechanisms account for AmpC activity in Escherichia coli, namely, mutations in the ampC promoter and attenuator regions resulting in ampC overexpression and acquisition of plasmid-carried ampC genes. In this study, we analyzed 51 clinical E. coli isolates with reduced susceptibility to amoxicillin-clavulanic acid, piperacillin-tazobactam, or extended-spectrum cephalosporins for the presence of AmpC production. Three phenotypic AmpC confirmation assays (cefoxitin-cloxacillin disk diffusion test, cefoxitin-EDTA disk diffusion test, and AmpC Etest) were compared for the detection of AmpC activity. All 51 isolates were characterized genetically by mutational analysis of the chromosomal ampC promoter/attenuator region and by PCR detection of plasmid-carried ampC genes. Altogether, 21/51 (41%) E. coli isolates were considered true AmpC producers. AmpC activity due to chromosomal ampC promoter/attenuator mutations was found in 12/21 strains, and plasmid-carried ampC genes were detected in 8/21 isolates. One strain contained both ampC promoter mutations and a plasmid-carried ampC gene. All three phenotypic tests were able to detect the majority (>90%) of AmpC-positive strains correctly. Cefoxitin resistance was found to be a discriminative parameter, detecting 20/21 AmpC-producing strains. Susceptibility to extended-spectrum cephalosporins, e.g., ceftriaxone, ceftazidime, and cefotaxime, was found in 9 of the 21 AmpC-positive strains. Considering the elevated zone diameter breakpoints of the 2010 CLSI guidelines, 2/21 AmpC-positive strains were categorized as susceptible to extended-spectrum cephalosporins.
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