ArticleJournal of clinical microbiology2011
Evaluation of high-resolution typing methods for Chlamydia trachomatis in samples from heterosexual couples.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 82 citations in OpenAlex.
- Diversity of Chlamydia trachomatis in Trachoma-Hyperendemic Communities Treated With Azithromycin.American journal of epidemiology · 2018Trial
- A cohort study of Chlamydia trachomatis treatment failure in women: a study protocol.BMC infectious diseases · 2013Trial
- High Prevalence and Genotypic Diversity of Persistent Chlamydia Trachomatis Infections Among South African Adolescent Girls and Young Women: A Tale of Two Cities.The Journal of infectious diseases · 2026Article
- High Prevalence of Haemophilus ducreyi Among Patients With Suspected Primary syphilis in Malawi, 2019-2022.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Unravelling Chlamydia trachomatis diversity in Amhara, Ethiopia: MLVA-ompA sequencing as a molecular typing tool for trachoma.PLoS neglected tropical diseases · 2024Article
- Genetic characterization of theParasitology · 2024Article
- Genomic insights into local-scale evolution of ocularMicrobial genomics · 2024Article
- Phenotypic and Genotypic Antimicrobial Susceptibility Testing ofAntibiotics (Basel, Switzerland) · 2023Article
- Genetic Characterization of Non-International journal of molecular sciences · 2023Article
- Whole Genome Sequencing of aCurrent issues in molecular biology · 2023Article
- Chronic epididymitis due toFrontiers in public health · 2023Article
- Evaluation ofJournal of tropical medicine · 2023Article
- Detection of Treponema pallidum DNA During Early Syphilis Stages in Peripheral Blood, Oropharynx, Ano-Rectum and Urine as a Proxy for Transmissibility.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Treponema pallidum SubspeciesMicrobiology spectrum · 2022Article
- Emergence of NovelMicroorganisms · 2022Article
- Specific high-resolution scheme to improve understanding of the spatio-temporal dispersion of lymphogranuloma venereum epidemic.Frontiers in microbiology · 2022Article
- Ongoing evolution ofMicrobial genomics · 2021Article
- Whole-Genome Enrichment and Sequencing of Chlamydia trachomatis Directly from Patient Clinical Vaginal and Rectal Swabs.mSphere · 2021Article
- High-resolution multilocus sequence typing for Chlamydia trachomatis: improved results for clinical samples with low amounts of C. trachomatis DNA.BMC microbiology · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We aimed to compare conventional ompA typing of Chlamydia trachomatis with multilocus sequence typing (MLST) and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA). Previously used MLST and MLVA systems were compared to modified versions that used shorter target regions and nested PCR. Heterosexual couples were selected from among persons with urogenital C. trachomatis infections visiting the sexually transmitted infection outpatient clinic in Amsterdam, The Netherlands. We identified 30 couples with a total of 65 C. trachomatis-positive samples on which MLST and MLVA for eight target regions were performed. All regions were successfully sequenced in 52 samples, resulting in a complete profile for 18 couples and 12 individuals. Nine ompA genovars from D to K, with two variants of genovar G, were found. The numbers of sequence type and MLVA type profiles were 20 for MLST and 21 for MLVA, and a combination of MLST and MLVA yielded 28 profiles, with discriminatory indexes (D) ranging from 0.95 to 0.99. Partners in 17 couples shared identical profiles, while partners in 1 couple had completely different profiles. Three persons had infections at multiple anatomical locations, and within each of these three individuals, all profiles were identical. The discriminatory capacity of all MLST and MLVA methods is much higher than that of ompA genotyping (D = 0.78). No genotype variation was found within the samples of the same person or from heterosexual couples with a putative single transmission. This shows that the chlamydial genome in clinical specimens has an appropriate polymorphism to enable epidemiological cluster analysis using MLST and MLVA.
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Registered trials
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