ArticleAmerican journal of physiology. Renal physiology2011
Activation of P2Y1 and P2Y2 receptors induces chloride secretion via calcium-activated chloride channels in kidney inner medullary collecting duct cells.
Article in American journal of physiology. Renal physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Purinergic Receptor P2Y2 Regulates Neurogenesis and Functional Recovery Following Spinal Cord Injury in Zebrafish.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Pharmacological Nature of the Purinergic P2Y Receptor Subtypes That Participate in the Blood Pressure Changes Produced by ADPβS in Rats.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Purinoceptor: a novel target for hypertension.Purinergic signalling · 2023Review
- Conjunctival Fluid Secretion Impairment via CaCC-CFTR Dysfunction Is the Key Mechanism in Environmental Dry Eye.International journal of molecular sciences · 2022Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Nociceptor Signalling through ion Channel Regulation via GPCRs.International journal of molecular sciences · 2019Review
- Orkambi® and amplifier co-therapy improves function from a rareEMBO molecular medicine · 2017Article
- Functional and therapeutic importance of purinergic signaling in polycystic kidney disease.American journal of physiology. Renal physiology · 2016Review
- A cAMP and CREB-mediated feed-forward mechanism regulates GSK3β in polycystic kidney disease.Journal of molecular cell biology · 2016Article
- New Insights into the Molecular Mechanisms Targeting Tubular Channels/Transporters in PKD Development.Kidney diseases (Basel, Switzerland) · 2016Review
- Chloride secretion by renal collecting ducts.Current opinion in nephrology and hypertension · 2015Article
- Harvest and primary culture of the murine aldosterone-sensitive distal nephron.American journal of physiology. Renal physiology · 2015Article
- Modulation of Chloride Channel Functions by the Plant Lignan Compounds Kobusin and Eudesmin.Frontiers in plant science · 2015Article
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- Prostaglandin E2 induces chloride secretion through crosstalk between cAMP and calcium signaling in mouse inner medullary collecting duct cells.American journal of physiology. Cell physiology · 2014Article
- P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica.PloS one · 2014Article
- Prostaglandin E(2) mediates proliferation and chloride secretion in ADPKD cystic renal epithelia.American journal of physiology. Renal physiology · 2012Article
- Differential effects of extracellular ATP on chloride transport in cortical collecting duct cells.American journal of physiology. Renal physiology · 2012Article
- Epithelial sodium channel regulation by cell surface-associated serum- and glucocorticoid-regulated kinase 1.The Journal of biological chemistry · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Dysregulation of urinary sodium chloride (NaCl) excretion can result in extracellular fluid (ECF) volume expansion and hypertension. Recent studies demonstrated that urinary nucleotide excretion increases in mice ingesting a high-salt diet and that these increases in extracellular nucleotides can signal through P2Y(2) receptors in the kidney collecting duct to inhibit epithelial Na(+) channels (ENaC). However, under conditions of ECF volume expansion brought about by high-dietary salt intake, ENaC activity should already be suppressed. We hypothesized that alternative pathways exist by which extracellular nucleotides control renal NaCl excretion. We used an inner medullary collecting duct (mIMCD-K2) cell line in an Ussing chamber system as a model to study additional ion transport pathways that are regulated by extracellular nucleotides. When ENaC was inhibited, the addition of adenosine triphosphate (ATP) to the basal side of cell sheets activated both P2Y(1) and P2Y(2) receptors, inducing a transient increase in short-circuit current (I(sc)); addition of ATP to the apical side activated only P2Y(2) receptors, inducing first a transient and then a sustained increase in I(sc). The ATP-induced increases in I(sc) were blocked by pretreatment with a phospholipase C (PLC) inhibitor, a calcium (Ca(2+)) chelator, or Ca(2+)-activated Cl(-) channel (CACC) inhibitors, suggesting that ATP signals through both PLC and intracellular Ca(2+) to activate CACC. We propose that P2Y(1) and P2Y(2) receptors operate in tandem in IMCD cells to provide an adaptive mechanism for enhancing urinary NaCl excretion in the setting of high-dietary NaCl intake.
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