ArticleNucleic acids research2011
Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- In situ sequence-specific visualization of single methylated cytosine on tissue sections using ICON probe and rolling-circle amplification.Histochemistry and cell biology · 2023Article
- The fragility of a structurally diverse duplication block triggers recurrent genomic amplification.Nucleic acids research · 2021Article
- Molecular Mechanisms ofFrontiers in oncology · 2020Review
- MYCN is amplified during S phase, and c‑myb is involved in controlling MYCN expression and amplification in MYCN‑amplified neuroblastoma cell lines.Molecular medicine reports · 2019Article
- Complex repeat structure promotes hyper-amplification and amplicon evolution through rolling-circle replication.Nucleic acids research · 2018Article
- SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes.Genome research · 2016Article
- Replication fork integrity and intra-S phase checkpoint suppress gene amplification.Nucleic acids research · 2015Article
- Complex genomic rearrangements at the PLP1 locus include triplication and quadruplication.PLoS genetics · 2015Article
- Homology-mediated end-capping as a primary step of sister chromatid fusion in the breakage-fusion-bridge cycles.Nucleic acids research · 2013Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells.
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Registered trials
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