ArticleApplied and environmental microbiology2011
Comparison of innovative molecular approaches and standard spore assays for assessment of surface cleanliness.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Metagenomic Methods for Addressing NASA's Planetary Protection Policy Requirements on Future Missions: A Workshop Report.Astrobiology · 2023Article
- Article
- Microscopic Characterization of Biological and Inert Particles Associated with Spacecraft Assembly Cleanroom.Scientific reports · 2019Article
- Article
- Cleanroom Maintenance Significantly Reduces Abundance but Not Diversity of Indoor Microbiomes.PloS one · 2015Article
- Article
- Quantification of encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods.PloS one · 2014Article
- Molecular and Phenetic Characterization of the Bacterial Assemblage of Hot Lake, WA, an Environment with High Concentrations of Magnesium Sulfate, and Its Relevance to Mars.International journal of astrobiology · 2014Article
- New perspectives on viable microbial communities in low-biomass cleanroom environments.The ISME journal · 2013Article
- Pyrosequencing-derived bacterial, archaeal, and fungal diversity of spacecraft hardware destined for Mars.Applied and environmental microbiology · 2012Article
- Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission.Astrobiology · 2012Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A bacterial spore assay and a molecular DNA microarray method were compared for their ability to assess relative cleanliness in the context of bacterial abundance and diversity on spacecraft surfaces. Colony counts derived from the NASA standard spore assay were extremely low for spacecraft surfaces. However, the PhyloChip generation 3 (G3) DNA microarray resolved the genetic signatures of a highly diverse suite of microorganisms in the very same sample set. Samples completely devoid of cultivable spores were shown to harbor the DNA of more than 100 distinct microbial phylotypes. Furthermore, samples with higher numbers of cultivable spores did not necessarily give rise to a greater microbial diversity upon analysis with the DNA microarray. The findings of this study clearly demonstrated that there is not a statistically significant correlation between the cultivable spore counts obtained from a sample and the degree of bacterial diversity present. Based on these results, it can be stated that validated state-of-the-art molecular techniques, such as DNA microarrays, can be utilized in parallel with classical culture-based methods to further describe the cleanliness of spacecraft surfaces.
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