ArticleApplied and environmental microbiology2011
Production in Escherichia coli of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with differing monomer compositions from unrelated carbon sources.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 102 citations in OpenAlex.
- Optimization of PHBV Synthesis Using Acetate as a Carbon Source by Paracoccus shandongensis wg2 via Response Surface Methodology.Current microbiology · 2026Article
- Metabolic Engineering Strategies for Enhanced Polyhydroxyalkanoate (PHA) Production inPolymers · 2025Review
- Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the ExtremophileMarine drugs · 2025Article
- Enhancement of polymyxin B1 production by an artificial microbial consortium ofSynthetic and systems biotechnology · 2024Article
- Copolymers and Blends Based on 3-Hydroxybutyrate and 3-Hydroxyvalerate Units.International journal of molecular sciences · 2023Review
- Bioprocess Engineering, Transcriptome, and Intermediate Metabolite Analysis of L-Serine High-YieldingMicroorganisms · 2022Article
- Transcriptome Analysis Reveals Potential Mechanisms of L-Serine Production byFoods (Basel, Switzerland) · 2022Article
- A Critical Review on the Economically Feasible and Sustainable Poly(3-Hydroxybutyrate-Polymers · 2022Review
- Coupled synthetic pathways improve the production of 3-hydroxypropionic acid in recombinantBiotechnology notes (Amsterdam, Netherlands) · 2022Article
- Construction of an Escherichia coli Strain Lacking Fimbriae by Deleting 64 Genes and Its Application for Efficient Production of Poly(3-Hydroxybutyrate) and l-Threonine.Applied and environmental microbiology · 2021Article
- Bio-based production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with modulated monomeric fraction in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) biopolymer by recombinant Bacillus megaterium in fed-batch bioreactors.Bioprocess and biosystems engineering · 2021Article
- Norwegian Soils and Waters Contain Mesophilic, Plastic-Degrading Bacteria.Microorganisms · 2021Article
- Improving L-serine formation by Escherichia coli by reduced uptake of produced L-serine.Microbial cell factories · 2020Article
- Biosynthesis of poly(3-hydroxybutyrate-3 Biotech · 2020Article
- Review
- Genome shuffling and high-throughput screening of Brevibacterium flavum MDV1 for enhanced L-valine production.World journal of microbiology & biotechnology · 2018Article
- Engineering Escherichia coli for efficient coproduction of polyhydroxyalkanoates and 5-aminolevulinic acid.Journal of industrial microbiology & biotechnology · 2018Article
- Genome features of moderately halophilic polyhydroxyalkanoate-producingStandards in genomic sciences · 2017Article
- Production of 3-Hydroxypropionic Acid via the Propionyl-CoA Pathway Using Recombinant Escherichia coli Strains.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The industrial production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) has been hindered by high cost and a complex control strategy caused by the addition of propionate. In this study, based on analysis of the PHBV biosynthesis process, we developed a PHBV biosynthetic pathway from a single unrelated carbon source via threonine biosynthesis in Escherichia coli. To accomplish this, we (i) overexpressed threonine deaminase, which is the key factor for providing propionyl-coenzyme A (propionyl-CoA), from different host bacteria, (ii) removed the feedback inhibition of threonine by mutating and overexpressing the thrABC operon in E. coli, and (iii) knocked out the competitive pathways of catalytic conversion of propionyl-CoA to 3-hydroxyvaleryl-CoA. Finally, we constructed a series of strains and mutants which were able to produce the PHBV copolymer with differing monomer compositions in a modified M9 medium supplemented with 20 g/liter xylose. The largest 3-hydroxyvalerate fraction obtained in the copolymer was 17.5 mol%.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.