ArticleNucleic acids research2011
High-level transgene expression by homologous recombination-mediated gene transfer.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus.Molecular biology of the cell · 2023Article
- Episomes and Transposases-Utilities to Maintain Transgene Expression from Nonviral Vectors.Genes · 2022Review
- Generation of a host cell line containing a MAR-rich landing pad for site-specific integration and expression of transgenes.Biotechnology progress · 2022Article
- Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells.Scientific reports · 2022Article
- A statistical framework for determination of minimal plasmid copy number required for transgene expression in mammalian cells.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- Genome-wide analysis of single nucleotide variants allows for robust and accurate assessment of clonal derivation in cell lines used to produce biologics.Biotechnology and bioengineering · 2020Article
- DNA barcoding reveals that injected transgenes are predominantly processed by homologous recombination in mouse zygote.Nucleic acids research · 2020Article
- Enhanced transgene expression using two β-globin MARs flanking expression cassettes in stably transfected CHO-K1 cells.3 Biotech · 2019Article
- Overexpression of a phospholipase (OsPLDα1) for drought tolerance in upland rice (Oryza sativa L.).Protoplasma · 2018Article
- Comparison of cell-based versus cell-free mammalian systems for the production of a recombinant human bone morphogenic growth factor.Engineering in life sciences · 2017Article
- MAR-Mediated transgene integration into permissive chromatin and increased expression by recombination pathway engineering.Biotechnology and bioengineering · 2017Article
- Assays for DNA double-strand break repair by microhomology-based end-joining repair mechanisms.Nucleic acids research · 2016Article
- Assessment of UCOE on Recombinant EPO Production and Expression Stability in Amplified Chinese Hamster Ovary Cells.Molecular biotechnology · 2015Article
- Cosmetics-triggered percutaneous remote control of transgene expression in mice.Nucleic acids research · 2015Article
- A role for homologous recombination proteins in cell cycle regulation.Cell cycle (Georgetown, Tex.) · 2015Article
- Engineering cells to improve protein expression.Current opinion in structural biology · 2014Review
- Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes.Nucleic acids research · 2014Article
- Combination of FACS and homologous recombination for the generation of stable and high-expression engineered cell lines.PloS one · 2014Article
- MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation.BMC molecular biology · 2013Article
- Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer.Transgenic research · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene transfer and expression in eukaryotes is often limited by a number of stably maintained gene copies and by epigenetic silencing effects. Silencing may be limited by the use of epigenetic regulatory sequences such as matrix attachment regions (MAR). Here, we show that successive transfections of MAR-containing vectors allow a synergistic increase of transgene expression. This finding is partly explained by an increased entry into the cell nuclei and genomic integration of the DNA, an effect that requires both the MAR element and iterative transfections. Fluorescence in situ hybridization analysis often showed single integration events, indicating that DNAs introduced in successive transfections could recombine. High expression was also linked to the cell division cycle, so that nuclear transport of the DNA occurs when homologous recombination is most active. Use of cells deficient in either non-homologous end-joining or homologous recombination suggested that efficient integration and expression may require homologous recombination-based genomic integration of MAR-containing plasmids and the lack of epigenetic silencing events associated with tandem gene copies. We conclude that MAR elements may promote homologous recombination, and that cells and vectors can be engineered to take advantage of this property to mediate highly efficient gene transfer and expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.