ArticleJournal of experimental botany2011
Functional characterization of BjCET3 and BjCET4, two new cation-efflux transporters from Brassica juncea L.
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 54 citations in OpenAlex.
- Molecular characterization and expression patterns of MTP genes under heavy metal stress in mustard (Brassica juncea L.).Scientific reports · 2024Article
- Cadmium Stress Signaling Pathways in Plants: Molecular Responses and Mechanisms.Current issues in molecular biology · 2024Review
- Metal tolerance gene family in barley: an in silico comprehensive analysis.Journal of applied genetics · 2023Article
- Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.BMC plant biology · 2022Article
- Identification and evolutionary analysis of the metal-tolerance protein family in eight Cucurbitaceae species.The plant genome · 2022Article
- Genome-Wide Identification, Structure Characterization, Expression Pattern Profiling, and Substrate Specificity of the Metal Tolerance Protein Family inPlants (Basel, Switzerland) · 2021Article
- Heavy Metal Transporters-Associated Proteins inGenes · 2020Article
- Article
- Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses.Frontiers in genetics · 2018Article
- Phytoextraction of Heavy Metals: A Promising Tool for Clean-Up of Polluted Environment?Frontiers in plant science · 2018Review
- Article
- Effect of Heavy Metals in Plants of the Genus Brassica.International journal of molecular sciences · 2015Review
- Cucumber metal transport protein MTP8 confers increased tolerance to manganese when expressed in yeast and Arabidopsis thaliana.Journal of experimental botany · 2014Article
- Characterization of a vacuolar zinc transporter OZT1 in rice (Oryza sativa L.).Molecular biology reports · 2013Article
- Application of glutathione to roots selectively inhibits cadmium transport from roots to shoots in oilseed rape.Journal of experimental botany · 2013Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brassica juncea is promising for metal phytoremediation, but little is known about the functional role of most metal transporters in this plant. The functional characterization of two B. juncea cation-efflux family proteins BjCET3 and BjCET4 is reported here. The two proteins are closely related to each other in amino acid sequence, and are members of Group III of the cation-efflux transporters. Heterologous expression of BjCET3 and BjCET4 in yeast confirmed their functions in exporting Zn, and possibly Cd, Co, and Ni. Yeast transformed with BjCET4 showed higher metal resistance than did BjCET3 transformed. The two BjCET-GFP fusion proteins were localized to the plasma membrane in the roots when expressed in tobacco, and significantly enhanced the plants' Cd tolerance ability. Under Cd stress, tobacco plants transformed with BjCET3 accumulated significant amounts of Cd in shoots, while maintaining similar shoot biomass production with vector-control subjects. Transformed BjCET4 tobacco plants showed significantly enhanced shoot biomass production with markedly decreased shoot Cd content. The two transporter genes have a lower basal transcript expression in B. juncea seedling tissues when grown in normal conditions than under metal-stress, however, their transcripts levels could be substantially increased by Zn, Cd, NaCl or PEG, suggesting that BjCET3 and BjCET4 may play roles in several stress conditions, roles which appear to be different from those of previous characterized cation-efflux transporters, for example, AtMTP1, BjCET2, and BjMTP1.
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