ArticleBiochemistry2011
Mutants of the base excision repair glycosylase, endonuclease III: DNA charge transport as a first step in lesion detection.
Article in Biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- The Influence of 2'-Deoxyguanosine Lesions on the Electronic Properties ofMolecules (Basel, Switzerland) · 2024Review
- Noncatalytic Domains in DNA Glycosylases.International journal of molecular sciences · 2022Review
- Biochemical and functional characterization of an endonuclease III from Thermococcus barophilus Ch5.World journal of microbiology & biotechnology · 2022Article
- Review
- The [4Fe4S] Cluster of Yeast DNA Polymerase ε Is Redox Active and Can Undergo DNA-Mediated Signaling.Journal of the American Chemical Society · 2021Article
- Redox Chemistry in the Genome: Emergence of the [4Fe4S] Cofactor in Repair and Replication.Annual review of biochemistry · 2019Review
- Effective Distance for DNA-Mediated Charge Transport between Repair Proteins.ACS central science · 2019Article
- Sensing DNA through DNA Charge Transport.ACS chemical biology · 2018Review
- Properties of Site-Specifically Incorporated 3-Aminotyrosine in Proteins To Study Redox-Active Tyrosines: Escherichia coli Ribonucleotide Reductase as a Paradigm.Biochemistry · 2018Article
- Electrical Probes of DNA-Binding Proteins.Methods in enzymology · 2017Article
- Redox Signaling through DNA.Israel journal of chemistry · 2016Article
- Cleavage and polyadenylation specificity factor 30: An RNA-binding zinc-finger protein with an unexpected 2Fe-2S cluster.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The current state of eukaryotic DNA base damage and repair.Nucleic acids research · 2015Review
- Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- UV radiation effects on a DNA repair enzyme: conversion of a [4Fe-4S](2+) cluster into a [2Fe-2S] (2+).Radiation and environmental biophysics · 2015Article
- DNA charge transport within the cell.Biochemistry · 2015Review
- Multiplexed electrochemistry of DNA-bound metalloproteins.Journal of the American Chemical Society · 2013Article
- DNA helicases associated with genetic instability, cancer, and aging.Advances in experimental medicine and biology · 2013Article
- MUTYH DNA glycosylase: the rationale for removing undamaged bases from the DNA.Frontiers in genetics · 2013Article
- Solution, surface, and single molecule platforms for the study of DNA-mediated charge transport.Physical chemistry chemical physics : PCCP · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Endonuclease III (EndoIII) is a base excision repair glycosylase that targets damaged pyrimidines and contains a [4Fe-4S] cluster. We have proposed a model where BER proteins that contain redox-active [4Fe-4S] clusters utilize DNA charge transport (CT) as a first step in the detection of DNA lesions. Here, several mutants of EndoIII were prepared to probe their efficiency of DNA/protein charge transport. Cyclic voltammetry experiments on DNA-modified electrodes show that aromatic residues F30, Y55, Y75, and Y82 help mediate charge transport between DNA and the [4Fe-4S] cluster. On the basis of circular dichroism studies to measure protein stability, mutations at residues W178 and Y185 are found to destabilize the protein; these residues may function to protect the [4Fe-4S] cluster. Atomic force microscopy studies furthermore reveal a correlation in the ability of mutants to carry out protein/DNA CT and their ability to relocalize onto DNA strands containing a single base mismatch; EndoIII mutants that are defective in carrying out DNA/protein CT do not redistribute onto mismatch-containing strands, consistent with our model. These results demonstrate a link between the ability of the repair protein to carry out DNA CT and its ability to relocalize near lesions, thus pointing to DNA CT as a key first step in the detection of base damage in the genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.