ArticleThe Journal of experimental medicine1990
Shedding of tumor necrosis factor receptors by activated human neutrophils.
Article in The Journal of experimental medicine, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed, 437 citations in OpenAlex.
- Induction of serum soluble tumor necrosis factor receptor II (sTNF-RII) and interleukin-1 receptor antagonist (IL-1ra) by interferon beta-1b in patients with progressive multiple sclerosis.Journal of neurology · 2008Trial
- Influence of pentoxifylline on cytokine levels and inflammatory parameters in septic shock.Intensive care medicine · 1996Trial
- Cytokine multimerization: when more is more and sometimes less.Nature reviews. Immunology · 2026Review
- The prognostic and predictive value of peripheral immune-related proteins in patients with lung cancer treated with radiotherapy.Frontiers in oncology · 2025Review
- Regulation of plasma soluble receptors of TNF and IL-1 in patients with COVID-19 differs from that observed in sepsis.The Journal of infection · 2024Article
- The rs11684747 and rs55790676 SNPs of ADAM17 influence tuberculosis susceptibility and plasma levels of TNF, TNFR1, and TNFR2.Frontiers in microbiology · 2024Article
- Molecular Basic of Pharmacotherapy of Cytokine Imbalance as a Component of Intervertebral Disc Degeneration Treatment.International journal of molecular sciences · 2023Review
- Severe acute pancreatitis exhibits distinct cytokine signatures and trajectories in humans: a prospective observational study.American journal of physiology. Gastrointestinal and liver physiology · 2022Observational
- High Levels of TNF-α and TIM-3 as a Biomarker of Immune Reconstitution Inflammatory Syndrome in People with HIV Infection.Life (Basel, Switzerland) · 2021Article
- The Clinical Significance of Increased Serum Proinflammatory Cytokines, C-Reactive Protein, and Erythrocyte Sedimentation Rate in Patients with Hidradenitis Suppurativa.Mediators of inflammation · 2017Article
- Biological Drugs in Guillain-Barré Syndrome: An Update.Current neuropharmacology · 2017Review
- Persistent p55TNFR expression impairs T cell responses during chronic tuberculosis and promotes reactivation.Scientific reports · 2016Article
- Baseline TNFα operational capacity in fetal and maternal circulation prior to the onset of labor: "tuned for different purposes".Reproductive sciences (Thousand Oaks, Calif.) · 2013Article
- Tumour necrosis factor receptor trafficking dysfunction opens the TRAPS door to pro-inflammatory cytokine secretion.Bioscience reports · 2012Review
- Characterization of TNF receptor subtype expression and signaling on pulmonary endothelial cells in mice.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- TNFR2 expression on non-bone marrow-derived cells is crucial for lipopolysaccharide-induced septic shock and downregulation of soluble TNFR2 level in serum.Cellular & molecular immunology · 2011Article
- Assessment of pre-diagnosis biomarkers of immune activation and inflammation: insights on the etiology of lymphoma.Journal of proteome research · 2011Review
- Relationship Between Neonatal Blood Protein Concentrations and Placenta Histologic Characteristics in Extremely Low GA Newborns.Pediatric research · 2011Article
- Molecular and cellular mechanisms of ectodomain shedding.Anatomical record (Hoboken, N.J. : 2007) · 2010Review
- Elevated endotoxin levels in non-alcoholic fatty liver disease.Journal of inflammation (London, England) · 2010Article
39 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capacity of human neutrophils (PMN) to bind tumor necrosis factor (TNF) was rapidly lost when the cells were incubated in suspension with agents that can stimulate their migratory and secretory responses. Both physiological (poly)peptides (FMLP, C5a, CSF-GM) and pharmacologic agonists (PMN, calcium ionophore A23187) induced the loss of TNF receptors (TNF-R) from the cell surface. Half-maximal loss in TNF-R ensued after only approximately 2 min with 10(-7) M FMLP at 37 degrees C, and required only 10(-9) M FMLP during a 30-min exposure. However, there were no such changes even with prolonged exposure of PMN to FMLP at 4 degrees or 16 degrees C. Scatchard analysis revealed loss of TNF-binding sites without change in their affinity (Kd approximately 0.4 nM) as measured at incompletely modulating concentrations of FMLP, C5a, PMA, or A23187. The binding of anti-TNF-R mAbs to PMN decreased in parallel, providing independent evidence for the loss of TNF-R from the cell surface. At the same time, soluble TNF-R appeared in the medium of stimulated PMN. This inference was based on the PMN- and FMLP-dependent generation of a nonsedimentable activity that could inhibit the binding of TNF to fresh human PMN or to mouse macrophages, and the ability of mAbs specific for human TNF-R to abolish inhibition by PMN-conditioned medium of binding of TNF to mouse macrophages. Soluble TNF-R activity was associated with a protein of Mr approximately 28,000 by ligand blot analysis of cell-free supernatants of FMLP-treated PMN. Thus, some portion of the FMLP-induced loss of TNF-R from human PMN is due to shedding of TNF-R. Shedding was unaffected by inhibitors of serine and thiol proteases and could not be induced with phosphatidylinositol-specific phospholipase C. Loss of TNF-R from PMN first stimulated by other agents may decrease their responsiveness to TNF. TNF-R shed by PMN may be one source of the TNF-binding proteins found in body fluids, and may blunt the actions of the cytokine on other cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.