ReviewProtein science : a publication of the Protein Society2011
The role of calcium ions in the stability and instability of a thermolysin-like protease.
Review in Protein science : a publication of the Protein Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- High-level expression and characterization of a thermostable thermolysin-like metalloprotease with industrial potential.Folia microbiologica · 2026Article
- Calcium activation mechanism of a noncanonical aromatic L-amino acid decarboxylase from psilocybin mushroom Psilocybe cubensis.Communications biology · 2026Article
- Botulinum Toxin Effects and Its Association with Vitamin and Mineral Supplementation: A Narrative Review.Nutrients · 2026Review
- Thermostable Proteases fromMicroorganisms · 2025Review
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- The Structure and Function of Biomaterial Endolysin EFm1 fromMaterials (Basel, Switzerland) · 2022Article
- Conformational remodeling enhances activity of lanthipeptide zinc-metallopeptidases.Nature chemical biology · 2022Article
- Insights into the Maturation of Pernisine, a Subtilisin-Like Protease from the Hyperthermophilic Archaeon Aeropyrum pernix.Applied and environmental microbiology · 2020Article
- Lysosomal protein thermal stability does not correlate with cellular half-life: global observations and a case study of tripeptidyl-peptidase 1.The Biochemical journal · 2020Article
- Optimization of Parameters that Affect the Activity of the Alkaline Protease from Halotolerant Bacterium,Iranian journal of biotechnology · 2016Article
- Proteases from Canavalia ensiformis: Active and Thermostable Enzymes with Potential of Application in Biotechnology.Biotechnology research international · 2016Article
- Mechanistic Insight into Trimethylamine N-Oxide Recognition by the Marine Bacterium Ruegeria pomeroyi DSS-3.Journal of bacteriology · 2015Article
- Ca K-edge XAS as a probe of calcium centers in complex systems.Inorganic chemistry · 2015Article
- Structural changes in halophilic and non-halophilic proteases in response to chaotropic reagents.The protein journal · 2014Article
- Signaling domain of Sonic Hedgehog as cannibalistic calcium-regulated zinc-peptidase.PLoS computational biology · 2014Article
- Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin.PLoS pathogens · 2014Article
- The C-terminal transmembrane domain of human phospholipid scramblase 1 is essential for the protein flip-flop activity and Ca²⁺-binding.The Journal of membrane biology · 2014Article
- Architecture and function of metallopeptidase catalytic domains.Protein science : a publication of the Protein Society · 2014Review
- Structure of gentlyase, the neutral metalloprotease of Paenibacillus polymyxa.Acta crystallographica. Section D, Biological crystallography · 2013Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermolysin and other secreted broad-specificity proteases, such as subtilisin or alpha-lytic protease, are produced as pre-pro-proteins that stay at least partially unfolded while in the cytosol. After secretion, the pro-proteases fold to their active conformations in a process that includes the autolytic removal of the pro-peptide. We review the life cycle of the thermolysin-like protease from Bacillus stearothermophilus in light of the calcium dependent stability and instability of the N-terminal domain. The protease binds calcium ions in the regions that are involved in the autolytic maturation process. It is generally assumed that the calcium ions contribute to the extreme stability of the protease, but experimental evidence for TLP-ste indicates that at least one of the calcium ions plays a regulatory role. We hypothesize that this calcium ion plays an important role as a switch that modulates the protease between stable and unstable states as appropriate to the biological need.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.