ArticleMolecules and cells2011
Identification of potential microRNAs and their targets in Brassica rapa L.
Article in Molecules and cells, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Predicting candidate miRNAs for targeting begomovirus to induce sequence-specific gene silencing in chilli plants.Frontiers in plant science · 2024Article
- Comprehensive In Silico Characterization and Expression Pro-Filing of DA1/DAR Family Genes inGenes · 2022Article
- Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis.BMC plant biology · 2022Article
- Identification of miRNA and their target genes inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- MiR1885 Regulates Disease Tolerance Genes inInternational journal of molecular sciences · 2021Article
- Genome wide in-silico miRNA and target network prediction from stress responsive Horsegram (Macrotyloma uniflorum) accessions.Scientific reports · 2020Article
- Identification of microRNAs and relative target genes in Moringa oleifera leaf and callus.Scientific reports · 2019Article
- Computational Identification of MicroRNAs and Their Transcript Target(s) in Field Mustard (Iranian journal of biotechnology · 2017Article
- High-resolution identification and abundance profiling of cassava (Manihot esculenta Crantz) microRNAs.BMC genomics · 2016Article
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- A genome-wide perspective of miRNAome in response to high temperature, salinity and drought stresses in Brassica juncea (Czern) L.PloS one · 2014Article
- Insilico profiling of microRNAs in Korean ginseng (Panax ginseng Meyer).Journal of ginseng research · 2013Article
- Computational identification of microRNAs and their targets in cassava (Manihot esculenta Crantz.).Molecular biotechnology · 2013Article
- In silico identification and characterization of conserved miRNAs and their target genes in sweet potato (Ipomoea batatas L.) expressed sequence tags (ESTs).Plant signaling & behavior · 2013Article
- Identification and profiling of novel microRNAs in the Brassica rapa genome based on small RNA deep sequencing.BMC plant biology · 2012Article
- Identification and validation of potential conserved microRNAs and their targets in peach (Prunus persica).Molecules and cells · 2012Article
- Circulating microRNAs as novel and sensitive biomarkers of acute myocardial Infarction.Clinical biochemistry · 2012Review
- Origins and evolution of microRNA genes in plant species.Genome biology and evolution · 2012Article
- Analysis of Arabidopsis transcription factor families revealed extensive capacity for cell-to-cell movement as well as discrete trafficking patterns.Molecules and cells · 2011Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are recently discovered, noncoding, small regulatory RNA molecules that negatively regulate gene expression. Although many miRNAs are identified and validated in many plant species, they remain largely unknown in Brassica rapa (AA 2n =, 20). B. rapa is an important Brassica crop with wide genetic and morphological diversity resulting in several subspecies that are largely grown for vegetables, oilseeds, and fodder crop production. In this study, we identified 186 miRNAs belonging to 55 families in B. rapa by using comparative genomics. The lengths of identified mature and pre-miRNAs ranged from 18 to 22 and 66 to 305 nucleotides, respectively. Comparison of 4 nucleotides revealed that uracil is the predominant base in the first position of B. rapa miRNA, suggesting that it plays an important role in miRNA-mediated gene regulation. Overall, adenine and guanine were predominant in mature miRNAs, while adenine and uracil were predominant in pre-miRNA sequences. One DNA sequence producing both sense and antisense mature miRNAs belonging to the BrMiR 399 family, which differs by 1 nucleotide at the, 20(th) position, was identified. In silico analyses, using previously established methods, predicted 66 miRNA target mRNAs for 33 miRNA families. The majority of the target genes were transcription factors that regulate plant growth and development, followed by a few target genes that are involved in fatty acid metabolism, glycolysis, biotic and abiotic stresses, and other cellular processes. Northern blot and qRT-PCR analyses of RNA samples prepared from different B. rapa tissues for 17 miRNA families revealed that miRNAs are differentially expressed both quantitatively and qualitatively in different tissues of B. rapa.
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