ArticlePloS one2011
SNP-based typing: a useful tool to study Bordetella pertussis populations.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Large- and small-scale population structure ofApplied and environmental microbiology · 2025Article
- Genome-Based Analysis of Aspergillus niger Aggregate Species from China and Their Potential for Fumonisin BCurrent microbiology · 2022Article
- Rapid and accurate SNP genotyping of clonal bacterial pathogens with BioHansel.Microbial genomics · 2021Article
- Article
- Rapid and simple SNP genotyping for Bordetella pertussis epidemic strain MT27 based on a multiplexed single-base extension assay.Scientific reports · 2021Article
- Genetic Diversity of ClinicalJournal of genomics · 2021Article
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- Article
- Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Studying Bordetella pertussis populations by use of SNPeX, a simple high-throughput single nucleotide polymorphism typing method.Journal of clinical microbiology · 2015Article
- Bordetella pertussis naturally occurring isolates with altered lipooligosaccharide structure fail to fully mature human dendritic cells.Infection and immunity · 2015Article
- Genetic Variation of Bordetella pertussis in Austria.PloS one · 2015Article
- Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959-2013.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Article
- Pertussis resurgence: waning immunity and pathogen adaptation - two sides of the same coin.Epidemiology and infection · 2014Review
- Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally.PloS one · 2014Article
- Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Development of a temperature-switch PCR-based SNP typing method for Mycobacterium ulcerans.PLoS neglected tropical diseases · 2012Article
- Article
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12 authors.
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Abstract
To monitor changes in Bordetella pertussis populations, mainly two typing methods are used; Pulsed-Field Gel Electrophoresis (PFGE) and Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA). In this study, a single nucleotide polymorphism (SNP) typing method, based on 87 SNPs, was developed and compared with PFGE and MLVA. The discriminatory indices of SNP typing, PFGE and MLVA were found to be 0.85, 0.95 and 0.83, respectively. Phylogenetic analysis, using SNP typing as Gold Standard, revealed false homoplasies in the PFGE and MLVA trees. Further, in contrast to the SNP-based tree, the PFGE- and MLVA-based trees did not reveal a positive correlation between root-to-tip distance and the isolation year of strains. Thus PFGE and MLVA do not allow an estimation of the relative age of the selected strains. In conclusion, SNP typing was found to be phylogenetically more informative than PFGE and more discriminative than MLVA. Further, in contrast to PFGE, it is readily standardized allowing interlaboratory comparisons. We applied SNP typing to study strains with a novel allele for the pertussis toxin promoter, ptxP3, which have a worldwide distribution and which have replaced the resident ptxP1 strains in the last 20 years. Previously, we showed that ptxP3 strains showed increased pertussis toxin expression and that their emergence was associated with increased notification in The Netherlands. SNP typing showed that the ptxP3 strains isolated in the Americas, Asia, Australia and Europe formed a monophyletic branch which recently diverged from ptxP1 strains. Two predominant ptxP3 SNP types were identified which spread worldwide. The widespread use of SNP typing will enhance our understanding of the evolution and global epidemiology of B. pertussis.
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