ArticleMolecular & cellular proteomics : MCP2011
Unraveling lactococcal phage baseplate assembly by mass spectrometry.
Article in Molecular & cellular proteomics : MCP, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Exploring Structural Diversity among Adhesion Devices Encoded by Lactococcal P335 Phages with AlphaFold2.Microorganisms · 2022Article
- Conserved and Diverse Traits of Adhesion Devices fromViruses · 2020Review
- Article
- Identification of Dual Receptor Binding Protein Systems in Lactococcal 936 Group Phages.Viruses · 2018Article
- Article
- Changes in protein structure monitored by use of gas-phase hydrogen/deuterium exchange.Proteomics · 2015Article
- Article
- Progress in lactic acid bacterial phage research.Microbial cell factories · 2014Review
- Molecular insights on the recognition of a Lactococcus lactis cell wall pellicle by the phage 1358 receptor binding protein.Journal of virology · 2014Article
- Probing bunyavirus N protein oligomerisation using mass spectrometry.Rapid communications in mass spectrometry : RCM · 2014Article
- Structures and host-adhesion mechanisms of lactococcal siphophages.Frontiers in microbiology · 2014Review
- Crystal structure of pb9, the distal tail protein of bacteriophage T5: a conserved structural motif among all siphophages.Journal of virology · 2014Article
- Using ion mobility spectrometry-mass spectrometry to decipher the conformational and assembly characteristics of the hepatitis B capsid protein.Biophysical journal · 2013Article
- Structure and functional analysis of the host recognition device of lactococcal phage tuc2009.Journal of virology · 2013Article
- A tale of a tail: Structural insights into the conformational properties of the polyglutamine protein ataxin-3.International journal of mass spectrometry · 2013Article
- Viral infection modulation and neutralization by camelid nanobodies.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Visualizing a complete Siphoviridae member by single-particle electron microscopy: the structure of lactococcal phage TP901-1.Journal of virology · 2013Article
- Review
- Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacteriophages belonging to the Caudovirales order possess a tail acting as a molecular machine used during infection to recognize the host and ensure high-efficiency genome delivery to the cell cytoplasm. They bear a large and sophisticated multiprotein organelle at their distal tail end, either a baseplate or a tail-tip, which is the control center for infectivity. We report here insights into the baseplate assembly pathways of two lactoccocal phages (p2 and TP901-1) using electrospray ionization-mass spectrometry. Based on our "block cloning" strategy we have expressed large complexes of their baseplates as well as several significant structural subcomplexes. Previous biophysical characterization using size-exclusion chromatography coupled with on-line light scattering and refractometry demonstrated that the overproduced recombinant proteins interact with each other to form large (up to 1.9 MDa) and stable assemblies. The structures of several of these complexes have been determined by x-ray diffraction or by electron microscopy. In this contribution, we demonstrate that electrospray ionization-mass spectrometry yields accurate mass measurements for the different baseplate complexes studied from which their stoichiometries can be discerned, and that the subspecies observed in the spectra provide valuable information on the assembly mechanisms of these large organelles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.