ArticleAntimicrobial agents and chemotherapy2011
Low fitness cost of the multidrug resistance gene cfr.
Article in Antimicrobial agents and chemotherapy, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 66 citations in OpenAlex.
- The pathogenicity and virulence of the opportunistic pathogenVirulence · 2024Review
- Ribosome-targeting antibiotics and resistanceRSC medicinal chemistry · 2023Review
- Occurrence ofAntibiotics (Basel, Switzerland) · 2023Article
- Antimicrobial Resistance in Campylobacter coli and Campylobacter jejuni from Human Campylobacteriosis in Taiwan, 2016 to 2019.Antimicrobial agents and chemotherapy · 2022Article
- Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.eLife · 2022Article
- Emergence ofAntibiotics (Basel, Switzerland) · 2020Article
- Article
- Device-Associated Meningitis by Linezolid-ResistantJournal of neurosciences in rural practice · 2019Article
- Linezolid and Rifampicin Combination to CombatFrontiers in microbiology · 2019Article
- A high incidence and coexistence of multiresistance genes cfr and optrA among linezolid-resistant enterococci isolated from a teaching hospital in Wenzhou, China.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Fate of antimicrobial resistance genes in response to application of poultry and swine manure in simulated manure-soil microcosms and manure-pond microcosms.Environmental science and pollution research international · 2017Article
- Combined Effect of the Cfr Methyltransferase and Ribosomal Protein L3 Mutations on Resistance to Ribosome-Targeting Antibiotics.Antimicrobial agents and chemotherapy · 2017Article
- Targeting Antibiotic Resistance.Angewandte Chemie (International ed. in English) · 2016Review
- Antimicrobial Resistance, Biofilm Formation and mecA Characterization of Methicillin-Susceptible S. aureus and Non-S. aureus of Beef Meat Origin in Egypt.Frontiers in microbiology · 2016Article
- Evolving resistance among Gram-positive pathogens.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2015Review
- Linezolid-resistant Staphylococcus aureus strain 1128105, the first known clinical isolate possessing the cfr multidrug resistance gene.Antimicrobial agents and chemotherapy · 2014Article
- Identification and characterization of linezolid-resistant cfr-positive Staphylococcus aureus USA300 isolates from a New York City medical center.Antimicrobial agents and chemotherapy · 2014Article
- High prevalence of Cfr-producing Staphylococcus species in retail meat in Guangzhou, China.BMC microbiology · 2014Article
- [Resistance to "last resort" antibiotics in Gram-positive cocci: The post-vancomycin era].Biomedica : revista del Instituto Nacional de Salud · 2014Review
- Aminoglycoside resistance 16S rRNA methyltransferases block endogenous methylation, affect translation efficiency and fitness of the host.RNA (New York, N.Y.) · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The recently described rRNA methyltransferase Cfr that methylates the conserved 23S rRNA residue A2503, located in a functionally critical region of the ribosome, confers resistance to an array of ribosomal antibiotics, including linezolid. A number of reports of linezolid-resistant cfr-positive clinical strains indicate the possible rapid spread of this resistance mechanism. Since the rate of dissemination and the efficiency of maintenance of a resistance gene depend on the fitness cost associated with its acquisition, we investigated the fitness cost of cfr expression in a laboratory Staphylococcus aureus strain. We found that acquisition of the cfr gene does not produce any appreciable reduction in the cell growth rate. Only in a cogrowth competition experiment was some loss of fitness observed because Cfr-expressing cells slowly lose to the cfr-negative control strain. Interestingly, cells expressing wild-type and catalytically inactive Cfr had very similar growth characteristics, indicating that the slight fitness cost associated with cfr acquisition stems from expression of the Cfr polypeptide rather than from the modification of the conserved rRNA residue. In some clinical isolates, cfr is coexpressed with the erm gene, which encodes a methyltransferase targeting another 23S rRNA residue, A2058. Dimethylation of A2058 by Erm notably increases the fitness cost associated with the Cfr-mediated methylation of A2503. The generally low fitness cost of cfr acquisition observed in our experiments with the laboratory S. aureus strain offers a microbiological explanation for the apparent spread of the cfr gene among pathogens.
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