ArticleStructure (London, England : 1993)2011
Domain orientation in the N-Terminal PDZ tandem from PSD-95 is maintained in the full-length protein.
Article in Structure (London, England : 1993), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Labelizer: systematic selection of protein residues for covalent fluorophore labeling.Nature communications · 2025Article
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Unmasking a two-faced protein.eLife · 2022Article
- Article
- Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.Chemphyschem : a European journal of chemical physics and physical chemistry · 2021Article
- Probing Interdomain Linkers and Protein Supertertiary Structure In Vitro and in Live Cells with Fluorescent Protein Resonance Energy Transfer.Journal of molecular biology · 2021Article
- Supertertiary protein structure affects an allosteric network.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Emerging Themes in PDZ Domain Signaling: Structure, Function, and Inhibition.International review of cell and molecular biology · 2019Review
- Identifying weak interdomain interactions that stabilize the supertertiary structure of the N-terminal tandem PDZ domains of PSD-95.Nature communications · 2018Article
- Electrostatic Interactions at the Dimer Interface Stabilize the E. coli β Sliding Clamp.Biophysical journal · 2017Article
- High Precision FRET at Single-molecule Level for Biomolecule Structure Determination.Journal of visualized experiments : JoVE · 2017Article
- Mechanistic basis of MAGUK-organized complexes in synaptic development and signalling.Nature reviews. Neuroscience · 2016Review
- Targeting protein-protein interactions with trimeric ligands: high affinity inhibitors of the MAGUK protein family.PloS one · 2015Article
- Structural insights into a wildtype domain of the oncoprotein E6 and its interaction with a PDZ domain.PloS one · 2013Article
- Six steps closer to FRET-driven structural biology.Nature methods · 2012Article
- A toolkit and benchmark study for FRET-restrained high-precision structural modeling.Nature methods · 2012Article
- Supertertiary structure of the synaptic MAGuK scaffold proteins is conserved.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Effect of Src kinase phosphorylation on disordered C-terminal domain of N-methyl-D-aspartic acid (NMDA) receptor subunit GluN2B protein.The Journal of biological chemistry · 2011Article
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4 authors.
Funding
Abstract
Tandem PDZ domains have been suggested to form structurally independent supramodules. However, dissimilarity between crystallography and NMR models emphasize their malleable conformation. Studies in full-length scaffold proteins are needed to examine the effect of tertiary interactions within their native context. Using single-molecule fluorescence to characterize the N-terminal PDZ tandem in PSD-95, we provide the first direct evidence that PDZ tandems can be structurally independent within a full-length scaffold protein. Molecular refinement using our data converged on a single structure with an antiparallel alignment of the ligand-binding sites. Devoid of interaction partners, single-molecule conditions captured PSD-95 in its unbound, ground state. Interactions between PDZ domains could not be detected while fluctuation correlation spectroscopy showed that other conformations are dynamically sampled. We conclude that ultra-weak interactions stabilize the conformation providing a "low-relief" energy landscape that allows the domain orientation to be flipped by environmental interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.