ArticleStructure (London, England : 1993)2011
Structural basis for catalytic activation of a serine recombinase.
Article in Structure (London, England : 1993), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Staphylococcal Cassette ChromosomeAntibiotics (Basel, Switzerland) · 2026Article
- Directed evolution on compact landing pads yields highly efficient recombinases for large DNA integration.Nucleic acids research · 2026Article
- Direct observation of subunit rotation during DNA strand exchange by serine recombinases.Nature communications · 2024Article
- Structural basis for topological regulation of Tn3 resolvase.Nucleic acids research · 2023Article
- Control of the Serine Integrase Reaction: Roles of the Coiled-Coil and Helix E Regions in DNA Site Synapsis and Recombination.Journal of bacteriology · 2021Article
- Snapshots of a molecular swivel in action.Nucleic acids research · 2018Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis.Nucleic acids research · 2017Article
- Crossover-site sequence and DNA torsional stress control strand interchanges by the Bxb1 site-specific serine recombinase.Nucleic acids research · 2016Article
- Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase.Scientific reports · 2016Article
- Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion.Nucleic acids research · 2015Article
- Nicked-site substrates for a serine recombinase reveal enzyme-DNA communications and an essential tethering role of covalent enzyme-DNA linkages.Nucleic acids research · 2015Article
- Serine Resolvases.Microbiology spectrum · 2015Review
- Mechanisms of DNA Transposition.Microbiology spectrum · 2015Review
- Site-specific DNA Inversion by Serine Recombinases.Microbiology spectrum · 2015Article
- Article
- Enhancing the specificity of recombinase-mediated genome engineering through dimer interface redesign.Journal of the American Chemical Society · 2014Article
- Expanding the zinc-finger recombinase repertoire: directed evolution and mutational analysis of serine recombinase specificity determinants.Nucleic acids research · 2014Article
- The ins and outs of serine integrase site-specific recombination.Current opinion in structural biology · 2014Review
- A proposed mechanism for IS607-family serine transposases.Mobile DNA · 2013Article
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5 authors.
Funding
Abstract
Sin resolvase is a site-specific serine recombinase that is normally controlled by a complex regulatory mechanism. A single mutation, Q115R, allows the enzyme to bypass the entire regulatory apparatus, such that no accessory proteins or DNA sites are required. Here, we present a 1.86 Å crystal structure of the Sin Q115R catalytic domain, in a tetrameric arrangement stabilized by an interaction between Arg115 residues on neighboring subunits. The subunits have undergone significant conformational changes from the inactive dimeric state previously reported. The structure provides a new high-resolution view of a serine recombinase active site that is apparently fully assembled, suggesting roles for the conserved active site residues. The structure also suggests how the dimer-tetramer transition is coupled to assembly of the active site. The tetramer is captured in a different rotational substate than that seen in previous hyperactive serine recombinase structures, and unbroken crossover site DNA can be readily modeled into its active sites.
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