ArticleTraffic (Copenhagen, Denmark)2011
Biochemical characterization of APPL endosomes: the role of annexin A2 in APPL membrane recruitment.
Article in Traffic (Copenhagen, Denmark), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Insulin receptor trafficking and interactions in muscle cells.Journal of the Endocrine Society · 2026Article
- ANXA2 Protein and Its Role in Neurodegeneration Processes.Life (Basel, Switzerland) · 2025Review
- Melanocytes in regenerative medicine applications and disease modeling.Journal of translational medicine · 2024Review
- Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosis.The Plant cell · 2023Article
- Revising Endosomal Trafficking under Insulin Receptor Activation.International journal of molecular sciences · 2021Review
- Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder.EBioMedicine · 2019Article
- The exocyst complex and Rab5 are required for abscission by localizing ESCRT III subunits to the cytokinetic bridge.Journal of cell science · 2019Article
- The mitochondrial metallochaperone SCO1 maintains CTR1 at the plasma membrane to preserve copper homeostasis in the murine heart.Human molecular genetics · 2017Article
- APPL1 is a multifunctional endosomal signaling adaptor protein.Biochemical Society transactions · 2017Review
- Complex Roles of Annexin A2 in Host Blood-Brain Barrier Invasion by Cryptococcus neoformans.CNS neuroscience & therapeutics · 2017Article
- Rab31 and APPL2 enhance FcγR-mediated phagocytosis through PI3K/Akt signaling in macrophages.Molecular biology of the cell · 2015Article
- APPL1 potentiates insulin sensitivity by facilitating the binding of IRS1/2 to the insulin receptor.Cell reports · 2014Article
- Annexin A2 mediates apical trafficking of renal Na⁺-K⁺-2Cl⁻ cotransporter.The Journal of biological chemistry · 2014Article
- Endosomal acidification by Na+/H+ exchanger NHE5 regulates TrkA cell-surface targeting and NGF-induced PI3K signaling.Molecular biology of the cell · 2013Article
- Annexin A2 heterotetramer: structure and function.International journal of molecular sciences · 2013Article
- APPL1 regulates basal NF-κB activity by stabilizing NIK.Journal of cell science · 2012Article
- Annexin A2 at the interface of actin and membrane dynamics: a focus on its roles in endocytosis and cell polarization.International journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
APPL endosomes are a recently identified subpopulation of early endosomes characterized by the presence of two homologous Rab5 effector proteins APPL1 and APPL2. They exhibit only limited colocalization with EEA1, another Rab5 effector and a marker of the canonical early endosomes. Although APPL endosomes appear to play important roles in cargo trafficking and signal transduction, their protein composition and biochemical properties remain largely unknown. Here we employed membrane fractionation methods to characterize APPL endosomes biochemically. We demonstrate that they represent heterogeneous membrane structures which can be discriminated from the canonical EEA1-positive early endosomes by their partly different physical properties and a distinct migration pattern in the continuous density gradients. In search for other potential markers of APPL endosomes we identified Annexin A2 as an interacting partner of both APPL1 and APPL2. Annexin A2 is a Ca(2+) and phosphatidylinositol 4,5-bisphosphate binding protein, previously implicated in several endocytic steps. We show that Annexin A2 co-fractionates and colocalizes with APPL endosomes. Moreover, silencing of its expression causes solubilization of APPL2 from endosomes. Although Annexin A2 is not an exclusive marker of APPL endosomes, our data suggest that it has an important function in membrane recruitment of APPL proteins, acting in parallel to Rab5.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.