ArticleFuture medicinal chemistry2011
Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.
Article in Future medicinal chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Harnessing Nature's Algorithm: From Test Tubes to Autonomous In Vivo Evolution.Biotechnology journal · 2026Review
- Directed Evolution of a Modular Polyketide Synthase Thioesterase for Generation of a Hybrid Macrocyclic Ring System.ACS catalysis · 2025Article
- Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity.Protein engineering, design & selection : PEDS · 2025Article
- Updated analysis to reject the laboratory-engineering hypothesis of SARS-CoV-2.Environmental research · 2023Article
- Designed High-Redox Potential Laccases Exhibit High Functional Diversity.ACS catalysis · 2022Article
- HyperXpress: Rapid Single Vessel DNA Assembly and Protein Production in Microliterscale.Frontiers in bioengineering and biotechnology · 2022Article
- MonokaryoticInternational journal of molecular sciences · 2021Article
- Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase.Computational and structural biotechnology journal · 2021Article
- Progress in Ameliorating Beneficial Characteristics of Microbial Cellulases by Genetic Engineering Approaches for Cellulose Saccharification.Frontiers in microbiology · 2020Review
- A Review of the Microbial Production of Bioactive Natural Products and Biologics.Frontiers in microbiology · 2019Review
- Computer-Guided Surface Engineering for Enzyme Improvement.Scientific reports · 2018Article
- Review: Engineering of thermostable enzymes for industrial applications.APL bioengineering · 2018Review
- Modification of fatty acid selectivity of Candida antarctica lipase A by error-prone PCR.Biotechnology letters · 2017Article
- Rosetta and the Design of Ligand Binding Sites.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Lectin engineering, a molecular evolutionary approach to expanding the lectin utilities.Molecules (Basel, Switzerland) · 2015Review
- Directed evolution of bacteriorhodopsin for applications in bioelectronics.Journal of the Royal Society, Interface · 2013Article
- Actinomycetes: a repertory of green catalysts with a potential revenue resource.BioMed research international · 2013Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
To address the synthesis of increasingly structurally diverse small-molecule drugs, methods for the generation of efficient and selective biological catalysts are becoming increasingly important. 'Directed evolution' is an umbrella term referring to a variety of methods for improving or altering the function of enzymes using a nature-inspired twofold strategy of mutagenesis followed by selection. This article provides an objective assessment of the effectiveness of directed evolution campaigns in generating enzymes with improved catalytic parameters for new substrates from the last decade, excluding studies that aimed to select for only improved physical properties and those that lack kinetic characterization. An analysis of the trends of methodologies and their success rates from 81 qualifying examples in the literature reveals the average fold improvement for k (cat) (or V (max)), K (m) and k (cat)/K (m) to be 366-, 12- and 2548-fold, respectively, whereas the median fold improvements are 5.4, 3 and 15.6. Further analysis by enzyme class, library-generation methodology and screening methodology explores relationships between successful campaigns and the methodologies employed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.