ArticlePlanta2011
Evolutionary significance of an algal gene encoding an [FeFe]-hydrogenase with F-domain homology and hydrogenase activity in Chlorella variabilis NC64A.
Article in Planta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
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- Energy metabolism and adaptation to hypoxia in the non-photosynthetic green alga Leontynka.BMC biology · 2026Article
- NativeInternational journal of molecular sciences · 2025Article
- Disrupted HPlant molecular biology · 2024Article
- Predicting the Structure of Enzymes with Metal Cofactors: The Example of [FeFe] Hydrogenases.International journal of molecular sciences · 2024Article
- The AlgaInternational journal of molecular sciences · 2023Article
- Increased Biomass and Polyhydroxybutyrate Production byInternational journal of molecular sciences · 2023Article
- Fantastic [FeFe]-Hydrogenases and Where to Find Them.Frontiers in microbiology · 2022Review
- Adaptation to life on land at high OProceedings. Biological sciences · 2019Article
- Flavodiiron-Mediated OPlant physiology · 2018Article
- Simultaneous quantum yield measurements of carbon uptake and oxygen evolution in microalgal cultures.PloS one · 2018Article
- Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002.Applied and environmental microbiology · 2015Article
- Characterization of Hydrogen Metabolism in the Multicellular Green Alga Volvox carteri.PloS one · 2015Article
- Evolution of acyl-ACP-thioesterases and β-ketoacyl-ACP-synthases revealed by protein-protein interactions.Journal of applied phycology · 2014Article
- Engineering Limonene and Bisabolene Production in Wild Type and a Glycogen-Deficient Mutant of Synechococcus sp. PCC 7002.Frontiers in bioengineering and biotechnology · 2014Article
- Evolutionary and biotechnological implications of robust hydrogenase activity in halophilic strains of Tetraselmis.PloS one · 2014Article
- De novo transcriptomic analysis of hydrogen production in the green alga Chlamydomonas moewusii through RNA-Seq.Biotechnology for biofuels · 2013Article
- Fermentation metabolism and its evolution in algae.Frontiers in plant science · 2013Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
[FeFe]-hydrogenases (HYDA) link the production of molecular H(2) to anaerobic metabolism in many green algae. Similar to Chlamydomonas reinhardtii, Chlorella variabilis NC64A (Trebouxiophyceae, Chlorophyta) exhibits [FeFe]-hydrogenase (HYDA) activity during anoxia. In contrast to C. reinhardtii and other chlorophycean algae, which contain hydrogenases with only the HYDA active site (H-cluster), C. variabilis NC64A is the only known green alga containing HYDA genes encoding accessory FeS cluster-binding domains (F-cluster). cDNA sequencing confirmed the presence of F-cluster HYDA1 mRNA transcripts, and identified deviations from the in silico splicing models. We show that HYDA activity in C. variabilis NC64A is coupled to anoxic photosynthetic electron transport (PSII linked, as well as PSII-independent) and dark fermentation. We also show that the in vivo H(2)-photoproduction activity observed is as O(2) sensitive as in C. reinhardtii. The two C. variabilis NC64A HYDA sequences are similar to homologs found in more deeply branching bacteria (Thermotogales), diatoms, and heterotrophic flagellates, suggesting that an F-cluster HYDA is the ancestral enzyme in algae. Phylogenetic analysis indicates that the algal HYDA H-cluster domains are monophyletic, suggesting that they share a common origin, and evolved from a single ancestral F-cluster HYDA. Furthermore, phylogenetic reconstruction indicates that the multiple algal HYDA paralogs are the result of gene duplication events that occurred independently within each algal lineage. Collectively, comparative genomic, physiological, and phylogenetic analyses of the C. variabilis NC64A hydrogenase has provided new insights into the molecular evolution and diversity of algal [FeFe]-hydrogenases.
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